LOCUS       sequence-28276-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: U6+27-20
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(267..284)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(267..283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(276..298)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(512..531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctggctttat atatcttgtg gaaaggacga aacaccgtgc tcgcttcggc agcacatata
       61 ctagtcgacg cggccgcctc gagccggtac ctgcactcgc gcactttacc ggtacctgca
      121 ctcgcgcact ttaccggtac ctgcactcgc gcactttacc ggtacctgca ctcgcgcact
      181 ttaccggatc gcgggcccgt ttaaacccgc tctagagcgg acttcggtcc gctttttact
      241 aggacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
      301 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      361 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      421 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt aaggtgatgg ttcacgtagt
      661 gggccatcgc cctgatagac ggatttttca ccctttgacg ttggantcna
//
