LOCUS       sequence-282765-        3398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1787
FEATURES             Location/Qualifiers
     source          1..3398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagcg tggcggggac gggggcgaga gtgaacgatt caacccgggg
       61 gagctcagga tggcccaaca gcaggccttg aggttccgag gtccggctcc cccaccaaat
      121 gcagtgatgc gaggcccacc acctctgatg cgacctcctc caccttttgg tatgatgcga
      181 ggccctcctc caccaccacg gccgcccttt ggacgtcctc cttttgatcc taatatgccg
      241 ccaatgcctc ctccaggagg gatacctcca cctatgggcc ctccacacct ccagagacca
      301 cctttcatgc ctcctcccat gagttccatg cctcctcctc cgggtatgat gtttccacca
      361 ggaatgcctc ctgtgactgc tcctggtact ccagcactac ctcctacgga ggagatatgg
      421 gttgaaaata aaactccaga tgggaaggtt tattattata atgctcggac acgtgaatct
      481 gcatggacca agccagatgg agttaaggtt attcagcaat cagaactgac acctatgctt
      541 gcagcccagg cacaggttca ggctcaggcc caggcgcagg ctcaggccca ggcgcaggct
      601 caggcccagg cacaagctca ggcccaggct caggctcagg cccaggccca ggcccaggcc
      661 caggcccagg cccaagccca agcccaggcc caggctcagg ctcaggcaca agctcaggcc
      721 caggcccagg ctcaggtcca ggcccaggtc caggcacaag tgcaagcaca agcagttgga
      781 gcttccaccc ctacgaccag tagcccagca cctgcagtat ccacttcaac atcatcatcc
      841 accccttcct ctaccacttc taccacaaca actgctactt cagttgcgca gacagtatca
      901 acacccacaa cacaagatca gaccccaagt tctgctgttt cagttgccac gcctacagtt
      961 agtgtttcaa ctcctgctcc tacagccaca cctgtgcaaa ccgttcccca gccgcaccct
     1021 cagacgttac ctcctgctgt tcctcattca gtacctcagc caacaacagc aatacctgct
     1081 tttccaccag taatggtacc tccgtttcgt gttccccttc ctggcatgcc aattccactt
     1141 ccaggtgtag caatgatgca aatagtcagc tgcccgtatg taaagacagt cgctaccacc
     1201 aagaccggtg tattgccagg aatggcccct cctatcgtac ccatgataca tccccaggtt
     1261 gctattgcag cttcacctgc taccttagct ggagcaacag cagtttctga atggactgaa
     1321 tataaaacag cagatgggaa gacatattat tataataata gaacattaga atcaacctgg
     1381 gaaaaacccc aagaactaaa ggaaaaagaa aagttagaag agaagattaa agagccaatt
     1441 aaagaaccct ctgaagagcc tctgccaatg gagacggagg aggaggatcc taaagaagag
     1501 cctataaagg agataaagga ggagcccaaa gaagaggaga tgactgaaga agaaaaggct
     1561 gcccagaagg caaagccagt tgctactgct cctattcctg gtactccatg gtgtgtcgtt
     1621 tggactggtg atgagcgggt cttcttttat aatcccacca ctcgtctttc tatgtgggac
     1681 cgacctgatg atctgattgg cagggcagat gttgacaaaa ttattcagga gccccctcat
     1741 aaaaaaggaa tggaggaatt gaagaaacta aggcacccaa ctccgacaat gctgtcgatc
     1801 caaaagtggc aattctctat gagtgcaatt aaagaggaac aagaattaat ggaagaaatt
     1861 aatgaagatg agcctgttaa agcaaaaaaa cggaagagag acgataataa agacattgac
     1921 tcagagaaag aagctgccat ggaagctgaa attaaagctg cccgagaaag ggccattgtc
     1981 cctctggagg ctcgaatgaa gcagttcaag gacatgctgc tagagagagg ggtgtctgct
     2041 ttttcaacgt gggagaagga gttgcacaag atagtttttg atccccggta cttacttctc
     2101 aatcctaaag agagaaaaca ggtgtttgat cagtatgtaa agaccagggc agaggaagaa
     2161 cgcagggaaa agaaaaataa aataatgcaa gccaaggaag atttcaaaaa aatgatggaa
     2221 gaagcaaaat ttaatccaag agcaactttt agtgaatttg cagccaagca tgctaaagat
     2281 tcaagattca aagcaattga aaagatgaaa gaccgagaag ccttgtttaa tgagtttgtg
     2341 gccgctgcta ggaagaaaga gaaagaagat tcgaagacca gaggtgagaa gattaaatcg
     2401 gatttctttg aactattatc taatcatcac ttggacagtc agtctcgatg gagcaaagta
     2461 aaagacaaag tagaaagtga tccacgttac aaagcagtag atagttcatc aatgagagaa
     2521 gaccttttca aacagtacat tgaaaaaata gccaagaatt tagactcaga aaaagaaaag
     2581 gagcttgaaa ggcaagcccg cattgaggca agccttcgag aacgagaaag ggaggttcaa
     2641 aaggcccgtt cagaacaaac aaaagaaata gatcgagaga gagagcagca caaacgagaa
     2701 gaagctatcc agaatttcaa agctcttctg tctgacatgg tacgttcttc agatgtgtca
     2761 tggtctgata ctcgtaggac cctccgaaaa gatcaccgct gggaatctgg atccttattg
     2821 gaaagagagg agaaagagaa gctttttaat gaacacattg aagcacttac caaaaaaaag
     2881 agagagcact ttaggcaact tctggatgaa acttctgcaa ttaccttaac atccacgtgg
     2941 aaagaagtaa aaaaaatcat taaggaagat cctcgatgta ttaagttctc ctccagtgac
     3001 aggaaaaaac aaagagaatt tgaagaatat atcagagaca aatatatcac agccaaagct
     3061 gacttcagga cgcttttgaa agagaccaaa tttataacat atagatccaa aaaattaatc
     3121 caagaatcag atcagcacct gaaagatgta gaaaaaattt tacagaatga caaacggtat
     3181 ctagtactgg actgtgtgcc agaggagagg cgtaaactga ttgtggcata tgttgatgac
     3241 ctggatcgcc ggggtccacc cccacctccc acagcatcgg agcccacgag acgatcaaca
     3301 aaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3361 tgtggaaagg acgactctaa ttaagccaca gaccaaga
//
