LOCUS       sequence-28277-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: U6+27-20pf
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(203..220)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(203..219)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(212..234)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      361..719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(448..467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     658..679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tggctttata tatcttgtgg aaaggacgaa acaccgtgct cgcttcggca gcacatatac
       61 tagtcgacgc ggccgcctcg agctacctgc actataagca ctttactacc tgcactataa
      121 gcactttagg gcccgtttaa acccgctcta gagcggactt cggtccgctt tttactagga
      181 cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      241 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      301 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
      361 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      421 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      481 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      541 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      601 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      661 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      721 aagg
//
