LOCUS       sequence-282771-        2621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1804
FEATURES             Location/Qualifiers
     source          1..2621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctctcac ccagggacag gtgacattca gggatgtggc tatagaattc
       61 tctcaggagg agtggacatg cctggacccc gctcagagga ctttgtacag ggacgtgatg
      121 ttggagaatt ataggaacct ggcctccctg ggaatctctt gttttgacct cagtattatc
      181 tctatgttgg agcaagggaa ggagcctttc actttggaga gccaagtaca aatagcagga
      241 aacccagatg gatgggaatg gatcaaagct gtgatcacag ctctctcttc cgaatttgta
      301 atgaaagatt tactacacaa agggaagagt aatacaggag aagtattcca aacagtgatg
      361 ttggaaagac aggaaagcca agacattgaa ggatgttcct tcagggaagt ccagaaaaat
      421 acacatggcc ttgagtatca atgcagagat gctgaaggaa attacaaagg agtgcttttg
      481 acccaagaag gcaatctcac tcatggaaga gatgaacatg ataaaagaga tgcaagaaac
      541 aagcttatta aaaatcagct tggattaagc cttcagtcac atctgcctga actgcagctt
      601 tttcaatatg aagggaaaat ttatgaatgt aatcaggttg agaagtcttt caacaataat
      661 tcctcagttt caccacctca acaaatgcct tataatgtca aaacccacat ttctaagaaa
      721 tatctcaaag attttatctc ttctttatta ctcacacagg ggcaaaaagc aaataattgg
      781 ggaagccctt acaaatctaa tggatgtggc atggtctttc ctcaaaattc acaccttgca
      841 agtcatcaga gaagtcatac taaagagaaa ccttacaaat gttatgagtg tggcaaagcc
      901 tttagaacac gttcaaacct aactacccat caggtgatcc atactggcga aaaacgttac
      961 aaatgtaatg agtgtggtaa ggtctttagt cgaaattcac aactctcaca acatcagaaa
     1021 attcacactg gagagaaacc ttataaatgt aacgaatgtg gcaaggtctt cactcagaat
     1081 tcacaccttg taagacatcg aggaattcat actggagaga aaccttacaa gtgtaatgag
     1141 tgtgggaaag cctttagagc tcgttcaagc ttagctatcc atcaggcaac ccacagtgga
     1201 gaaaaacctt acaaatgtaa tgaatgtggc aaggtcttca ctcaaaattc acaccttaca
     1261 aatcactgga gaattcacac tggagagaaa ccttacaagt gtaatgagtg cggcaaagcc
     1321 tttggtgttc gttcaagcct agctattcat ctggtaattc acaccggaga aaagccttac
     1381 aaatgtcatg aatgcggcaa ggtctttagg cgtaattcac accttgcaag gcatcagcta
     1441 attcatactg gagagaaacc ttataagtgt aatgagtgtg gcaaagcctt tagagcacat
     1501 tcaaacctaa ctacccatca ggtcatccat actggagaaa agccttacaa atgtaatgaa
     1561 tgtggcaagg tcttcactca aaattcacac cttgcaaatc atcaaagaat tcatactgga
     1621 gtgaagcctt atatgtgtaa tgagtgcggc aaagccttca gtgtgtattc aagcctaact
     1681 acccatcagg tcatccatac tggagaaaaa ccttacaaat gtaatgagtg tggcaaggtc
     1741 ttcactcaga attcacacct tgcaagacat cggggaattc atactggaga gaaaccttat
     1801 aaatgtaatg aatgtggcaa ggtctttagg cataattcat acctttcaag gcatcagcga
     1861 attcatactg gagagaaacc ttacaagtat aatgagtatg gcaaagcctt tagtgaacat
     1921 tcaaacctaa ctacccatca ggtcatccat actggtgaaa aaccttacaa atgtaatgag
     1981 tgtggcaagg tcttcactca gaattcacac cttgcaagac atcggagagt tcatactgga
     2041 ggtaaacctt accagtgtaa tgaatgtggc aaagccttta gtcaaacatc aaagcttgca
     2101 aggcatcaga gagttcatac tggagagaaa ccatatgagt gtaatcagtg tgggaaagcc
     2161 tttagtgtcc gttcaagcct aactacccat caggcaatcc atactggaaa aaaaccttac
     2221 aaatgcaatg agtgtgggaa ggtcttcact cagaattcac accttgcaag acatcgggga
     2281 attcatactg gagagaaacc ttacaagtgt aatgagtgtg gcaaagcctt tagtcaaact
     2341 tcaaaacttg caaggcatca gagaattcat accggagaga aaccatatga gtgtgggaaa
     2401 ccctttagta tctgttcaag cctaactacc catcagacaa tccatactgg tgggaaacct
     2461 tacaaatgta acgtgtggaa agttctaaag tcagagttca aaccttgcaa gccatcacag
     2521 aattcatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2581 tcttgtggaa aggacgaggc cctaacaccg cccgttcgac c
//
