LOCUS       sequence-282777-        2015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1860
FEATURES             Location/Qualifiers
     source          1..2015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             325..354
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             328..357
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             331..360
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             334..363
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             337..366
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tgcatgactc aatcagattt ggaaatgtgg atatgccctg cgtccaagcc
       61 caatatagcc cttcccctcc aggttccagt tatgcggcgc agacatacag ctcggaatac
      121 accacggaga tcatgaaccc cgactacacc aagctgacca tggaccttgg cagcactgag
      181 atcacggcta cagccaccac gtccctgccc agcatcagta ccttcgtgga gggctactcg
      241 agcaactacg aactcaagcc ttcctgcgtg taccaaatgc agcggccctt gatcaaagtg
      301 gaggaggggc gggcgcccag ctaccatcac catcaccacc accaccacca ccaccaccac
      361 catcaccagc agcagcatca gcagccatcc attcctccag cctccagccc ggaggacgag
      421 gtgctgccca gcacctccat gtacttcaag cagtccccac cgtccacccc caccacgccg
      481 gccttccccc cgcaggcggg ggcgttatgg gacgaggcac tgccctcggc gcccggctgc
      541 atcgcacccg gcccgctgct ggacccgccg atgaaggcgg tccccacggt ggccggcgcg
      601 cgcttcccgc tcttccactt caagccctcg ccgccgcatc cccccgcgcc cagcccggcc
      661 ggcggccacc acctcggcta cgacccgacg gccgctgccg cgctcagcct gccgctggga
      721 gccgcagccg ccgcgggcag ccaggccgcc gcgcttgaga gccacccgta cgggctgccg
      781 ctggccaaga gggcggcccc gctggccttc ccgcctctcg gcctcacgcc ctcccctacc
      841 gcgtccagcc tgctgggcga gagtcccagc ctgccgtcgc cgcccagcag gagctcgtcg
      901 tctggcgagg gcacgtgtgc cgtgtgcggg gacaacgccg cctgccagca ctacggcgtg
      961 cgaacctgcg agggctgcaa gggctttttc aagagaacag tgcagaaaaa tgcaaaatat
     1021 gtttgcctgg caaataaaaa ctgcccagta gacaagagac gtcgaaaccg atgtcagtac
     1081 tgtcgatttc agaagtgtct cagtgttgga atggtaaaag aagttgtccg tacagatagt
     1141 ctgaaaggga ggagaggtcg tctgccttcc aaaccaaaga gcccattaca acaggaacct
     1201 tctcagccct ctccaccttc tcctccaatc tgcatgatga atgcccttgt ccgagcttta
     1261 acagactcaa cacccagaga tcttgattat tccagatact gtcccactga ccaggctgct
     1321 gcaggcacag atgctgagca tgtgcaacaa ttctacaacc tcctgacagc ctccattgat
     1381 gtatccagaa gctgggcaga aaagattccg ggatttactg atctccccaa agaagatcag
     1441 acattactta ttgaatcagc ctttttggag ctgtttgtcc tcagactttc catcaggtca
     1501 aacactgctg aagataagtt tgtgttctgc aatggacttg tcctgcatcg acttcagtgc
     1561 cttcgtggat ttggggagtg gctcgactct attaaagact tttccttaaa tttgcagagc
     1621 ctgaaccttg atatccaagc cttagcctgc ctgtcagcac tgagcatgat cacagaaaga
     1681 catgggttaa aagaaccaaa gagagtcgaa gagctatgca acaagatcac aagcagttta
     1741 aaagaccacc agagtaaggg acaggctctg gagcccaccg agtccaaggt cctgggtgcc
     1801 ctggtagaac tgaggaagat ctgcaccctg ggcctccagc gcatcttcta cctgaagctg
     1861 gaagacttgg tgtctccacc ttccatcatt gacaagctct tcctggacac cctacctttc
     1921 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1981 ggaaaggacg actgttgagg acagtggagc cctct
//
