LOCUS       sequence-282780-        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1864
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggttcactc cagcatgggg gctccagaaa taagaatgtc taagcccctg
       61 gaggccgaga agcaaggtct ggactcccca tcagagcaca cagacaccga aagaaatgga
      121 ccagacacta atcatcagaa cccccaaaat aagacctccc cattctccgt gtccccaact
      181 ggccccagta caaagatcaa ggctgaagac cccagtggcg attcagcccc agcagcaccc
      241 ctgccccctc agccggccca gcctcatctg ccccaggccc aactcatgtt gacgggcagc
      301 cagctagctg gggacataca gcagctcctc cagctccagc agctggtgct tgtgccaggc
      361 caccacctcc agccacctgc tcagttcctg ctaccgcagg cccagcagag ccagccaggc
      421 ctgctaccga caccaaatct attccagcta cctcagcaaa cccagggagc tcttctgacc
      481 tcccagcccc gggccgggct tcccacacag gccgtgaccc gccctacgct gcccgacccg
      541 cacctctcgc acccgcagcc ccccaaatgc ttggagccac catcccaccc cgaggagccc
      601 agtgatctgg aggagctgga gcaattcgcc cgcaccttca agcaacgccg catcaagctg
      661 ggcttcacgc agggtgatgt gggcctggcc atgggcaagc tctacggcaa cgacttcagc
      721 cagacgacca tttcccgctt cgaggccctc aacctgagct tcaagaacat gtgcaaactc
      781 aagcccctcc tggagaagtg gctcaacgat gcagagacta tgtctgtgga ctcaagcctg
      841 cccagcccca accagctgag cagccccagc ctgggtttcg acggcctgcc cggccggaga
      901 cgcaagaaga ggaccagcat cgagacaaac gtccgcttcg ccttagagaa gagttttcta
      961 gcgaaccaga agcctacctc agaggagatc ctgctgatcg ccgagcagct gcacatggag
     1021 aaggaagtga tccgcgtctg gttctgcaac cggcgccaga aggagaaacg catcaacccc
     1081 tgcagtgcgg cccccatgct gcccagccca gggaagccgg ccagctacag cccccatatg
     1141 gtcacacccc aagggggcgc ggggacctta ccgttgtccc aagcttccag cagtctgagc
     1201 acaacagtta ctaccttatc ctcagctgtg gggacgctcc accccagccg gacagctgga
     1261 gggggtgggg gcgggggcgg ggctgcgccc cccctcaatt ccatcccctc tgtcactccc
     1321 ccacccccgg ccaccaccaa cagcacaaac cccagccctc aaggcagcca ctcggctatc
     1381 ggcttgtcag gcctgaaccc cagcacgggc cctggcctct ggtggaaccc tgccccttac
     1441 cagccttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1501 tcttgtggaa aggacgaacg tcaaatactt atggagacgt a
//
