LOCUS       sequence-282783-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1866
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggttcactc cagcatgggg gctccagaaa taagaatgtc taagcccctg
       61 gaggccgaga agcaaggtct ggactcccca tcagagcaca cagacaccga aagaaatgga
      121 ccagacacta atcatcagaa cccccaaaat aagacctccc cattctccgt gtccccaact
      181 ggccccagta caaagatcaa ggctgaagac cccagtggcg attcagcccc agcagcaccc
      241 ctgccccctc agccggccca gcctcatctg ccccaggccc aactcatgtt gacgggcagc
      301 cagctagctg gggacataca gcagctcctc cagctccagc agctggtgct tgtgccaggc
      361 caccacctcc agccacctgc tcagttcctg ctaccgcagg cccagcagag ccagccaggc
      421 ctgctaccga caccaaatct attccagcta cctcagcaaa cccagggagc tcttctgacc
      481 tcccagcccc gggccgggct tcccacacag ccccccaaat gcttggagcc accatcccac
      541 cccgaggagc ccagtgatct ggaggagctg gagcaattcg cccgcacctt caagcaacgc
      601 cgcatcaagc tgggcttcac gcagggtgat gtgggcctgg ccatgggcaa gctctacggc
      661 aacgacttca gccagacgac catttcccgc ttcgaggccc tcaacctgag cttcaagaac
      721 atgtgcaaac tcaagcccct cctggagaag tggctcaacg atgcagagac tatgtctgtg
      781 gactcaagcc tgcccagccc caaccagctg agcagcccca gcctgggttt cgacggcctg
      841 cccggccgga gacgcaagaa gaggaccagc atcgagacaa acgtccgctt cgccttagag
      901 aagagttttc tagcgaacca gaagcctacc tcagaggaga tcctgctgat cgccgagcag
      961 ctgcacatgg agaaggaagt gatccgcgtc tggttctgca accggcgcca gaaggagaaa
     1021 cgcatcaacc cctgcagtgc ggcccccatg ctgcccagcc cagggaagcc ggccagctac
     1081 agcccccata tggtcacacc ccaagggggc gcggggacct taccgttgtc ccaagcttcc
     1141 agcagtctga gcacaacagt tactacctta tcctcagctg tggggacgct ccaccccagc
     1201 cggacagctg gagggggtgg gggcgggggc ggggctgcgc cccccctcaa ttccatcccc
     1261 tctgtcactc ccccaccccc ggccaccacc aacagcacaa accccagccc tcaaggcagc
     1321 cactcggcta tcggcttgtc aggcctgaac cccagcacgg gccctggcct ctggtggaac
     1381 cctgcccctt accagcctta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgac tgacgtttga ttccgccgtg gca
//
