LOCUS       sequence-282795-        2699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1925
FEATURES             Location/Qualifiers
     source          1..2699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggatt actgcattac atcaaccctg cacacgccat ttctctccta
       61 agtgccctga atgaggagcg tctcaaagga cagctgtgtg atgtgctgct gattgttgga
      121 gaccaaaagt tccgagctca taaaaacgtc ttggctgcca gcagcgaata ctttcagagt
      181 ttattcacaa ataaggaaaa tgagtcacaa actgtatttc agcttgactt ctgtgagcca
      241 gatgcttttg ataatgtttt aaactacatt tattcttcct ctctatttgt tgagaagagc
      301 agccttgctg ctgtgcaaga acttggctat agtcttggga tttcctttct gactaacatc
      361 gtttctaaaa cacctcaagc cccctttcca acgtgtccta atagaaaaaa agtgtttgta
      421 gaagatgatg aaaacagttc tcaaaagaga agtgtcattg tttgtcaaag tagaaacgaa
      481 gcgcaaggaa aaactgttag tcaaaatcaa cccgatgtaa gccatacttc ccggccctct
      541 cctagcattg cagtcaaggc caataccaat aagccacatg tcccaaaacc aatagaacca
      601 cttcataatt tgtcattaac tgaaaagagt tggccgaaag atagttctgt ggtatatgca
      661 aagtctcttg agcattctgg atctttggat gatcctaata gaatcagttt ggtgaaaaga
      721 aatgcagtgt tgccttcaaa gcctctgcaa gacagagaag ctatggatga taaaccaggt
      781 gtgagtggtc agcttccaaa aggaaaagct ctagagctgg ctttgaagag accacggcca
      841 cctgttttgt ctgtttgtag ctcatcagag actccctatc tattaaaaga aactaacaaa
      901 ggaaatggtc aaggtgaaga tagaaacttg ttgtactatt caaagttagg cttagtgatc
      961 ccatccagtg gatctggttc tggaaaccaa agcattgaca ggagtggccc acttgttaag
     1021 agtctcctca gacggtcatt gtcgatggat agccaggttc ctgtctattc accttccata
     1081 gatttgaaat cttcccaggg atcatcttcg gtgtccagtg atgcaccagg gaatgtgttg
     1141 tgtgctttat ctcagaagtc atctttaaaa gattgtagtg aaaaaacagc cctagatgac
     1201 aggcctcaag tgctacaacc gcatcgcctc aggtccttta gtgcttctca gtcaacagac
     1261 agggagggag cttcccctgt gactgaggtg cgcataaaga ctgagcccag cagcccgctg
     1321 tcggacccct cggacatcat ccgcgtcact gtgggagatg cggcaacaac agcagctgcc
     1381 tcatcttcgt cggtcacaag agacctgtct ctgaaaacag aagatgacca aaaagacatg
     1441 agcagactcc cagcaaaaag gaggttccaa gcggaccgaa gattgccgtt taagaagtta
     1501 aaggtgaatg agcacgggtc tcctgtgtca gaagataatt ttgaggaagg ctcaagccct
     1561 actctccttg atgcagattt tccagattct gatttgaata aagacgaatt tgagcaagga
     1621 agccacgagc ggctgtgccg gaacgcggcc gtctgccctt actgcagcct caggtttttc
     1681 tcgcccgagc tgaagcaaga acacgagagc aagtgtgagt ataagaagct gacctgcctc
     1741 gagtgcatgc gcaccttcaa gtcctctttc agcatctggc ggcaccaggt tgaagtccat
     1801 aatcagaaca acatggcacc caccgaaaac ttttctttgc ccgttttgga ccacaatggt
     1861 gatgtgactg gttcttcaag gccccaatcc cagcctgagc ccaacaaagt aaaccacatc
     1921 gtcaccacaa aagacgacaa cgtgttcagt gattcttcag aacaagttaa cttcgactcg
     1981 gaagattcct cttgtcttcc cgaagacctt agtctttcca agcaactgaa aatccaagtc
     2041 aaagaggagc ctgtggagga ggctgaagaa gaggcacccg aggccagcac agcccccaaa
     2101 gaagcgggtc ctagcaaaga agccagcctg tggccctgcg agaagtgtgg gaagatgttc
     2161 acggtgcata agcagctgga gcgtcaccag gagcttctgt gctctgtgaa accatttatt
     2221 tgtcacgtgt gcaacaaagc ttttcgcact aattttcgac tctggagtca cttccaatcg
     2281 cacatgtctc aggcttcaga ggaatcggca cataaggaat ctgaggtgtg ccctgttccc
     2341 acaaactctc cctctccacc acctctgcca ccgccaccac cactgcccaa gatccagcct
     2401 ctggagcctg acagccccac aggcctgtcc gaaaacccaa ctccagccac agaaaaactg
     2461 tttgtgcccc aagaatcaga cacccttttt taccatgccc cacccctttc agcaatcaca
     2521 tttaaaagac agtttatgtg taaactttgc cacaggacat tcaagactgc atttagtctt
     2581 tggagtcacg aacaaacaca caattagtaa tgaactagtc cgtaacttga aagtatttcg
     2641 atttcttggc tttatatatc ttgtggaaag gacgacaaga tatgggttaa gcctgtccc
//
