LOCUS       sequence-282798-        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1927
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgatgag agagctgaac gcggattcat gctcaagccc ccagatgggg
       61 gctatgtggg agactagtgg gtctgtgaaa gagaactcca gtcagagtaa gaaatacagc
      121 acaaaaatag agaatcttgg tcctgagagc gcttgcaggc acttctggag cttccgttat
      181 catgaagcaa ccggaccgct tgagactatc agccaacttc agaaattgtg ccatcagtgg
      241 ctgaggccag agatccactc aaaagagcag atcttggaaa tgctggtgtt agagcagttc
      301 ctgagcattc tgcccaagga gacccagaac tgggtgcaga agcatcatcc acagaatgtc
      361 aaacaggctc tggtcctggt ggaattcttg cagagggagc ctgatggaac aaagaatgag
      421 agtttgttga catttgaaga tgtggctgtg tatttttctg aggaagagtg gcaattattg
      481 aatcctcttg agaagactct ctacaatgat gtaatgcagg atatctatga gactgtcatc
      541 tctctagggt taaagctaaa aaatgacact ggaaatgatc atcctatatc tgtttctaca
      601 tcagaaatac aaacatcagg atgcgaagta tcaaaaaaga ccagaatgaa aattgcccag
      661 aaaacaatgg gcagggaaaa tcctggtgat acacacagtg tacagaaatg gcatcgagct
      721 tttccaagga agaaaagaaa gaaacctgca acttgtaaac aagagcttcc aaaacttatg
      781 gatcttcatg ggaaaggccc cacaggggag aaacctttta agtgtcagga atgtgggaaa
      841 agcttcagag ttagctctga tcttattaaa caccacagaa ttcacactgg agagaaaccc
      901 tataaatgtc aacaatgtga caggaggttt agatggagtt cagatcttaa taagcacttc
      961 atgacccatc aaggaataaa accatataga tgctcatggt gtgggaaaag ctttagtcat
     1021 aacacaaatc tacacacaca ccaaagaatt cacacaggag agaagccctt taaatgtgat
     1081 gaatgtggaa aaagattcat tcagaactcc caccttatta aacaccagag aactcacaca
     1141 ggtgagcagc cttatacgtg tagcttatgc aagagaaact ttagtaggcg atcgagcctt
     1201 cttagacacc agaaactcca cagaagaagg gaagcatgtc tagtgtctcc aaactagtaa
     1261 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1321 gacgatcacg actctcgttt cagcagtcc
//
