LOCUS       sequence-28280-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Ebert MS, Neilson JR, Sharp PA
  TITLE     MicroRNA sponges: competitive inhibitors of small RNAs in mammalian 
            cells.
  JOURNAL   Nat Methods. 2007 Sep . 4(9):721-6.
  PUBMED    17694064
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Sep . 4(9):721-6."
COMMENT     Sequence Label: U6+27-cxcr4
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(268..284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(277..299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      426..784
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(513..532)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     723..744
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtgctcgct tcggcagcac
       61 atatactagt cgacgcggcc gcctcgagcc ggaagttttc agaaagctaa caccggaagt
      121 tttcagaaag ctaacaccgg aagttttcag aaagctaaca ccggaagttt tcagaaagct
      181 aacaccggat cgcgggcccg tttaaacccg ctctagagcg gacttcggtc cgctttttac
      241 taggacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
      301 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      361 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      421 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      481 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      541 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      601 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      661 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      721 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      781 ttta
//
