LOCUS       sequence-282807-        1739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1949
FEATURES             Location/Qualifiers
     source          1..1739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaggc ctcctcgctg gggaggcaga gtcctcgcgt ggtctcctgc
       61 ctcgagcaca gcctgtgccc aggggagccg ggcttgcaga caacagcagt ggtgtccatg
      121 ggctctggag accatcagtt caacctcgca gagatcctgt cacagaacta cagtgttagg
      181 ggggagtgcg aggaggcctc gaggtgccca gacaagccca aggaggagct ggagaaggac
      241 ttcatctccc agagcaacga catgcccttt gatgagctgc ttgcgctcta tggctacgag
      301 gcgtcagacc ccatttcaga ccgggagagt gagggtggtg acgtggcccc gaacctccca
      361 gacatgaccc tggacaaaga acaaatagcg aaggatttgc tttcagggga agaagaggaa
      421 gagacgcaat catctgctga cgacctcacc ccgtccgtga cctcccacga ggcctccgac
      481 ctcttcccta accggagtgg atctcgtttc ctggctgatg aagacagaga gcctggctct
      541 tctgcctcct ccgacaccga ggaggactct cttcctgcca acaaatgtaa gaaggagatc
      601 atggtgggac ctcagttcca agctgacctc agcaacctgc acttgaaccg gcactgtgag
      661 aagatctacg agaacgaaga ccagctgctc tgggacccca gcgtcctccc tgagagggag
      721 gtggaggagt tcctgtacag ggcggtgaag cggcgttggc acgagatggc cgggcctcag
      781 ctcccagagg gagaagccgt gaaagacagt gagcaggcgc tgtacgagtt ggtgaaatgc
      841 aacttcaatg tggaggaggc cctgcgaagg ctgcggttca acgtgaaggt gatccgagat
      901 gggctctgtg cttggagtga agaggagtgc aggaactttg agcacggctt ccgtgtgcat
      961 ggaaagaact ttcacctgat ccaggccaac aaggtgcgca cacggtcagt gggcgagtgt
     1021 gtcgagtact actacctgtg gaagaagtcg gagcgctacg actacttcgc ccagcagacg
     1081 cggctgggcc ggaggaagta cgtcccgtcc ggaaccacgg acgcagacca ggacctggat
     1141 ggcagcgacc ccgatggccc cggccgtccg cgcccggagc aagacaccct gactgggatg
     1201 cgcacagatc cactgagcgt ggatggcacg gccggtggtc tcgatgagcc cggagtggcc
     1261 tctgatggac tcccgtcctc ggagccaggg ccgtgttcct tccagcagct ggatgagtcc
     1321 cccgctgtac ccctgtccca tcggccccca gccctggccg acccagcctc ataccagcca
     1381 gctgtcactg ctccggagcc agacgccagc ccaaggctgg ccgtggactt cgccctgccc
     1441 aaggagctgc ccctcatctc cagccatgtg gacctcagcg gggatccgga ggagactgtg
     1501 gccccagcac aggtggcttt gtcggtcacc gagtttggac tcatcggcat tggggacgtg
     1561 aaccccttcc tggccgccca ccccacgtgc ccggcccccg ggctacactc ggagcccctg
     1621 tcacactgta acgtgatgac ctgctagtaa tgaactagtc cgtaacttga aagtatttcg
     1681 atttcttggc tttatatatc ttgtggaaag gacgaataag agacgacggt gccatatat
//
