LOCUS       sequence-282810-        1427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1965
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcagcc acaggaggaa gcccctgagg tccgggaaga ggaggagaaa
       61 gaggaagtgg cagaggcaga aggagcccca gagctcaatg ggggaccaca gcatgcactt
      121 ccttccagca gctacacaga cctctcccgg agctcctcgc caccctcact gctggaccaa
      181 ctgcagatgg gctgtgacgg ggcctcatgc ggcagcctca acatggagtg ccgggtgtgc
      241 ggggacaagg catcgggctt ccactacggt gttcatgcat gtgaggggtg caagggcttc
      301 ttccgtcgta cgatccgcat gaagctggag tacgagaagt gtgagcgcag ctgcaagatt
      361 cagaagaaga accgcaacaa gtgccagtac tgccgcttcc agaagtgcct ggcactgggc
      421 atgtcacaca acgctatccg ttttggtcgg atgccggagg ctgagaagag gaagctggtg
      481 gcagggctga ctgcaaacga ggggagccag tacaacccac aggtggccga cctgaaggcc
      541 ttctccaagc acatctacaa tgcctacctg aaaaacttca acatgaccaa aaagaaggcc
      601 cgcagcatcc tcaccggcaa agccagccac acggcgccct ttgtgatcca cgacatcgag
      661 acattgtggc aggcagagaa ggggctggtg tggaagcagt tggtgaatgg cctgcctccc
      721 tacaaggaga tcagcgtgca cgtcttctac cgctgccagt gcaccacagt ggagaccgtg
      781 cgggagctca ctgagttcgc caagagcatc cccagcttca gcagcctctt cctcaacgac
      841 caggttaccc ttctcaagta tggcgtgcac gaggccatct tcgccatgct ggcctctatc
      901 gtcaacaagg acgggctgct ggtagccaac ggcagtggct ttgtcacccg tgagttcctg
      961 cgcagcctcc gcaaaccctt cagtgatatc attgagccta agtttgaatt tgctgtcaag
     1021 ttcaacgccc tggaacttga tgacagtgac ctggccctat tcattgcggc catcattctg
     1081 tgtggagacc ggccaggcct catgaacgtt ccacgggtgg aggctatcca ggacaccatc
     1141 ctgcgtgccc tcgaattcca cctgcaggcc aaccaccctg atgcccagta cctcttcccc
     1201 aagctgctgc agaagatggc tgacctgcgg caactggtca ccgagcacgc ccagatgatg
     1261 cagcggatca agaagaccga aaccgagacc tcgctgcacc ctctgctcca ggagatctac
     1321 aaggacatgt actagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1381 tatatatctt gtggaaagga cgattggaat tgatttctct gacatcc
//
