LOCUS       sequence-282813-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1968
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             217..246
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             220..249
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tggcctcggg cgacaccctc tacatcgcca cggacggctc ggagatgccg
       61 gccgagatcg tggagctgca cgagatcgag gtggagacca tcccggtgga gaccatcgag
      121 accacagtgg tgggcgagga ggaggaggag gacgacgacg acgaggacgg cggcggtggc
      181 gaccacggcg gcgggggcgg ccacgggcac gccggccacc accaccacca ccatcaccac
      241 caccaccacc cgcccatgat cgctctgcag ccgctggtca ccgacgaccc gacccaggtg
      301 caccaccacc aggaggtgat cctggtgcag acgcgcgagg aggtggtggg cggcgacgac
      361 tcggacgggc tgcgcgccga ggacggcttc gaggatcaga ttctcatccc ggtgcccgcg
      421 ccggccggcg gcgacgacga ctacattgaa caaacgctgg tcaccgtggc ggcggccggc
      481 aagagcggcg gcggcggctc gtcgtcgtcg ggaggcggcc gcgtcaagaa gggcggcggc
      541 aagaagagcg gcaagaagag ttacctcagc ggcggggccg gcgcggcggg cggcggcggc
      601 gccgacccgg gcaacaagaa gtgggagcag aagcaggtgc agatcaagac cctggagggc
      661 gagttctcgg tcaccatgtg gtcctcagat gaaaaaaaag atattgacca tgagacagtg
      721 gttgaagaac agatcattgg agagaactca cctcctgatt attcagaata tatgacagga
      781 aagaaacttc ctcctggagg aatacctggc attgacctct cagatcccaa acaactggca
      841 gaatttgcta gaatgaagcc aagaaaaatt aaagaagatg atgctccaag aacaatagct
      901 tgccctcata aaggctgcac aaagatgttc agggataact cggccatgag aaaacatctg
      961 cacacccacg gtcccagagt ccacgtctgt gcagaatgtg gcaaagcttt tgttgagagt
     1021 tcaaaactaa aacgacacca actggttcat actggagaga agccctttca gtgcacgttc
     1081 gaaggctgtg ggaaacgctt ttcactggac ttcaatttgc gcacacatgt gcgaatccat
     1141 accggagaca ggccctatgt gtgccccttc gatggttgta ataagaagtt tgctcagtca
     1201 actaacctga aatctcacat cttaacacat gctaaggcca aaaacaacca gtagtaatga
     1261 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1321 gaataagtta tcactcgagc cgatcg
//
