LOCUS       sequence-282816-        1658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1983
FEATURES             Location/Qualifiers
     source          1..1658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgacgc agacgagggc tttggcctgg cgcacacgcc tctggagcct
       61 gacgcaaaag acctgccctg cgattcgaaa cccgagagcg cgctcggggc ccccagcaag
      121 tccccgtcgt ccccgcaggc cgccttcacc cagcagggca tggagggaat caaagtgttt
      181 ctccatgaaa gagaactgtg gctaaaattc cacgaagtgg gcacggaaat gatcataacc
      241 aaggctggaa ggcggatgtt tcccagttac aaagtgaagg tgacgggcct taatcccaaa
      301 acgaagtaca ttcttctcat ggacattgta cctgccgacg atcacagata caaattcgca
      361 gataataaat ggtctgtgac gggcaaagct gagcccgcca tgcctggccg cctgtacgtg
      421 cacccagact cccccgccac cggggcgcat tggatgaggc agctcgtctc cttccagaaa
      481 ctcaagctca ccaacaacca cctggaccca tttgggcata ttattctaaa ttccatgcac
      541 aaataccagc ctagattaca catcgtgaaa gcggatgaaa ataatggatt tggctcaaaa
      601 aatacagcgt tctgcactca cgtctttcct gagactgcgt ttatagcagt gacttcctac
      661 cagaaccaca agatcacgca attaaagatt gagaataatc cctttgccaa aggatttcgg
      721 ggcagtgatg acatggagct gcacagaatg tcaagaatgc aaagtaaaga atatcccgtg
      781 gtccccagga gcaccgtgag gcaaaaagtg gcctccaacc acagtccttt cagcagcgag
      841 tctcgagctc tctccacctc atccaatttg gggtcccaat accagtgtga gaatggtgtt
      901 tccggcccct cccaggacct cctgcctcca cccaacccat acccactgcc ccaggagcat
      961 agccaaattt accattgtac caagaggaaa gaggaagaat gttccaccac agaccatccc
     1021 tataagaagc cctacatgga gacatcaccc agtgaagaag attccttcta ccgctctagc
     1081 tatccacagc agcagggcct gggtgcctcc tacaggacag agtcggcaca gcggcaagct
     1141 tgcatgtatg ccagctctgc gccccccagc gagcctgtgc ccagcctaga ggacatcagc
     1201 tgcaacacgt ggccaagcat gccttcctac agcagctgca ccgtcaccac cgtgcagccc
     1261 atggacaggc taccctacca gcacttctcc gctcacttca cctcggggcc cctggtccct
     1321 cggctggctg gcatggccaa ccatggctcc ccacagctgg gagagggaat gttccagcac
     1381 cagacctccg tggcccacca gcctgtggtc aggcagtgtg ggcctcagac tggcctgcag
     1441 tcccctggca cccttcagcc ccctgagttc ctctactctc atggcgtgcc aaggactcta
     1501 tcccctcatc agtaccactc tgtgcacgga gttggcatgg tgccagagtg gagcgacaat
     1561 agctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1621 tgtggaaagg acgaacctca cacagggtga agcatgga
//
