LOCUS       sequence-282819-        1901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1989
FEATURES             Location/Qualifiers
     source          1..1901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc actgagggcc ccgacccagc aggtttttgt agcctttgag
       61 gatgtggcca tttacttctc ccaggaggag tgggagctcc ttgatgagat gcagaggctc
      121 ctgtaccgcg atgtgatgct ggagaacttt gcagttatgg catccctagg ttgctggtgt
      181 ggagcagtag atgaggggac gccttctgca gagagcgttt ctgtggaaga actgtcacag
      241 ggcaggactc caaaggcaga tacatccact gataagagtc acccctgtga gatttgtacc
      301 ccagtcctga gagacatttt acaaatgatt gagctccatg cctcaccctg tggacagaaa
      361 ttgtacttgg gtggagcatc aagagatttc tggatgagtt caaaccttca ccagctccag
      421 aagcttgata atggagagaa gctctttaaa gtggatgggg accaggcctc atttatgatg
      481 aactgcaggt tccatgtgtc aggaaaaccc ttcacgtttg gggaagtcgg gagggacttt
      541 tcagccacct caggacttct ccagcatcag gtgactccca ccattgagag accacacagc
      601 aggattagac acttgagagt tcccactgga cgaaagcctc tcaaatacac tgaatccagg
      661 aaatctttta gagagaaatc tgtattcatt caacaccaaa gagctgactc tggagaaagg
      721 ccttacaagt gcagtgaatg tgggaaatcc tttagtcaaa gttctggctt tcttcgacac
      781 aggaaagcac acggtagaac aaggactcat gaatgtagtg aatgtgggaa atcatttagt
      841 cgcaaaactc acctaactca acaccaaaga gttcacactg gagaaaggcc ttatgactgc
      901 agtgaatgtg gcaaatcctt tcgccaggta tctgtcctca ttcaacatca acgagttcac
      961 actggagaaa ggccttatga gtgcagtgaa tgtgggaaat cttttagcca cagcactaac
     1021 ctctatcgtc acaggagtgc ccacactagc acaaggcctt atgagtgcag tgaatgtgga
     1081 aaatccttta gccatagcac taacctcttt cgacactgga gagttcacac tggagtaagg
     1141 ccttatgagt gtagtgaatg tgggaaagca tttagttgca atatctacct tattcaccac
     1201 caaagatttc acactggaga aagaccttat gtgtgcagtg aatgtgggaa atcatttggc
     1261 cagaaatctg tcctcattca acaccaaaga gttcacactg gagaaaggcc ttatgagtgc
     1321 agtgaatgtg ggaaagtttt tagccaaagc tctggcctct ttcgacacag aagagctcac
     1381 actaaaacaa agccttatga gtgcagtgaa tgtgaaaaat catttagttg caaaactgac
     1441 ctcattcgac accagacagt tcacactgga gaaaggcctt atgagtgcag tgtatgtggg
     1501 aaatctttta tccgaaaaac ccacctcatt cgacaccaga ctgttcacac taatgaaagg
     1561 ccttatgagt gcgatgaatg tgggaaatcc tatagccaaa gctctgccct ccttcagcat
     1621 aggagagttc acactggaga aaggccttat gagtgcagag aatgtgggaa atcttttacc
     1681 cgcaaaaatc acctcattca acacaagaca gttcacactg gagaaaggcc ttatgaatgc
     1741 agtgaatgtg gaaaatcctt tagccaaagc tctggcctct taagacacag aagagttcat
     1801 gtgcagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1861 tcttgtggaa aggacgatta atacagatct aataagctca c
//
