LOCUS       sequence-282822-        2615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1991
FEATURES             Location/Qualifiers
     source          1..2615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctagcaa acgaaaatct acaactccat gcatggttcg gacatcacaa
       61 gtagtagaac aagatgtgcc cgaggaagta gacagggcca aagagaaagg aatcggcaca
      121 ccacagcctg acgtggccaa ggacagttgg gcagcagaac ttgaaaactc ttccaaagaa
      181 aacgaagtga tagaggtgaa atctatgggg gaaagccagt ccaaaaaact ccaaggtggt
      241 tatgagtgca aatactgccc ctactccacg caaaacctga acgagttcac ggagcatgtc
      301 gacatgcagc atcccaacgt gattctcaac cccctctacg tgtgtgcaga atgtaacttc
      361 acaaccaaaa agtacgactc cctatccgac cacaactcca agttccatcc cggggaggcc
      421 aacttcaagc tgaagttaat taaacgcaat aatcaaactg tcttggaaca gtccatcgaa
      481 accaccaacc atgtcgtgtc catcaccacc agtggccctg gaactggtga cagtgattct
      541 gggatctcgg tgagtaaaac ccccatcatg aagcctggaa aaccaaaagc ggatgccaag
      601 aaggtgccca agaagcccga ggagatcacc cccgagaacc acgtggaagg gaccgcccgc
      661 ctggtgacag acacagctga gatcctctcg agactcggcg gggtggagct cctccaagac
      721 acattaggac acgtcatgcc ttctgtacag ctgccaccaa atatcaacct tgtgcccaag
      781 gtccctgtcc cactaaatac taccaaatac aactctgccc tggatacaaa tgccacgatg
      841 atcaactctt tcaacaagtt tccttacccg acccaggctg agttgtcctg gctgacagct
      901 gcctccaaac acccagagga gcacatcaga atctggtttg ccacccagcg cttaaagcat
      961 ggcatcagct ggtccccaga agaggtggag gaggcccgga agaagatgtt caacggcacc
     1021 atccagtcag tacccccgac catcactgtg ctgcccgccc agttggcccc cacaaaggtg
     1081 acgcagccca tcctccagac ggctctaccg tgccagatcc tcggccagac tagcctggtg
     1141 ctgactcagg tgaccagcgg gtcaacaacc gtctcttgct cccccatcac acttgccgtg
     1201 gcaggagtca ccaaccatgg ccagaagaga cccttggtga ctccccaagc tgcccccgaa
     1261 cccaagcgtc cacacatcgc tcaggtgcca gagcccccac ccaaggtggc caaccccccg
     1321 ctcacaccag ccagtgaccg caagaagaca aaggagcaga tagcacatct caaggccagc
     1381 tttctccaga gccagttccc tgacgatgcc gaggtttacc ggctcatcga ggtgactggc
     1441 cttgccagga gcgagatcaa gaagtggttc agtgaccacc gatatcggtg tcaaaggggc
     1501 atcgtccaca tcaccagcga atcccttgcc aaagaccagt tggccatcgc ggcctcccga
     1561 cacggtcgca cgtatcatgc gtacccagac tttgcccccc agaagttcaa agagaaaaca
     1621 cagggtcagg ttaaaatctt ggaagacagc tttttgaaaa gttcttttcc tacccaagca
     1681 gaactggatc ggctaagggt ggagaccaag ctgagcagga gagagatcga ctcctggttc
     1741 tcggagaggc ggaagcttcg agacagcatg gaacaagctg tcttggattc catggggtct
     1801 ggcaaaaaag gccaagatgt gggagccccc aatggtgctc tgtctcgact cgaccagctc
     1861 tccggtgccc agttaacaag ttctctgccc agcccttcgc cagcaattgc aaaaagtcaa
     1921 gaacaggttc atctcctgag gagcacgttt gcaagaaccc agtggcctac tccccaggag
     1981 tacgaccagt tagcggccaa gactggcctg gtccgaactg agattgtgcg ttggttcaag
     2041 gagaacagat gcttgctgaa aacgggaacc gtgaagtgga tggagcagta ccagcaccag
     2101 cccatggcag atgatcacgg ctacgatgcc gtagcaagga aagcaacaaa acccatggcc
     2161 gagagcccaa agaacggggg tgatgtggtt ccacaatatt acaaggaccc caaaaagctc
     2221 tgcgaagagg acttggagaa gttggtgacc agggtaaaag taggcagcga gccagcaaaa
     2281 gactgtttgc cagcaaagcc ctcagaggcc acctcagacc ggtcagaggg cagcagccgg
     2341 gacggccagg gtagcgacga gaacgaggag tcgagcgttg tggattacgt ggaggtgacg
     2401 gtcggggagg aggatgcgat ctcagataga tcagatagct ggagtcaggc tgcggcagaa
     2461 ggtgtgtcgg aactggctga atcagactcc gactgcgtcc ctgcagaggc tggccaggcc
     2521 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2581 ggaaaggacg aaagagagtc ggccctacgc gatga
//
