LOCUS       sequence-28283-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, 
            Katovich MJ, Semple-Rowland SL, Raizada MK
  TITLE     Efficient large-scale production and concentration of HIV-1-based 
            lentiviral vectors for use in vivo.
  JOURNAL   Physiol Genomics. 2003 Feb 6. 12(3):221-8.
  PUBMED    12488511
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Physiol 
            Genomics. 2003 Feb 6. 12(3):221-8."
COMMENT     Sequence Label: pCEP4-tat
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      289..298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(348..372)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(369..572)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(398..418)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        573..876
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctgtctctgt ctctctctcc nccttcttct tcgattccgt gggccctgtc gggtcccctc
       61 gggactggga ggcgggttgc tttgatagag aagcttgatg agtctgactg tcctgaggag
      121 ctcttcgtcg ctgtctccgc ttcttcctgc cataggagat gcctaagcct tttcttgtga
      181 aacacgcgta gcaatgaaag caacactttt tacaatagca attgttacaa gcagtcctag
      241 gctgacttcc tggatgcttc cagggctcta gattaggatc tactggctcc atggtggctc
      301 gagcggccgc tagcaagctt gctagcagct ggtacccagc ttctagagat ctgacggttc
      361 actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
      421 aacggggcgg ggttattacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
      481 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
      541 cgcccattgg tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
      601 atgtactgcc aagtaggaaa gtcccgtaag gtcatgtact gggcataatg ccaggcgggc
      661 catttaccgt cattgacgtc aatagggggc ggacttggca tatgatacac ttgatgtact
      721 gncaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
      781 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
      841 cagccaggcg ggncatttac cgtaagttat gtaacgcgga nt
//
