LOCUS       sequence-282846-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2157
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcgttc gctcgtccac gacaccgtgt tctactgcct gagtgtatac
       61 caggtaaaaa taagccccac acctcagctg ggggcagcat caagcgcaga aggccatgtt
      121 ggccaaggag ctccaggcct catgggtaat atgaaccctg agggcggtgt gaaccacgag
      181 aacggcatga accgcgatgg cggcatgatc cccgagggcg gcggtggaaa ccaggagcct
      241 cggcagcagc cgcagccccc gccggaggag ccggcccagg cggccatgga gggtccgcag
      301 cccgagaaca tgcagccacg aactcggcgc acgaagttca cgctgttgca ggtggaggag
      361 ctggaaagtg ttttccgaca cactcaatac cctgatgtgc ccacaagaag ggaacttgcc
      421 gaaaacttag gtgtgactga agacaaagtg cgggtttggt ttaagaataa aagggccaga
      481 tgtaggcgac atcagagaga attaatgctc gccaatgaac tacgtgctga cccagacgac
      541 tgtgtctaca tcgtcgtgga ctagtaatga actagtccgt aacttgaaag tatttcgatt
      601 tcttggcttt atatatcttg tggaaaggac gaggcaagac gagcgaatga ttcctc
//
