LOCUS       sequence-282849-        1826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2181
FEATURES             Location/Qualifiers
     source          1..1826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcggc tgcgccgagg cgcccgactc agggcactgt gacctttgaa
       61 gatgtggctg tgaacttttc ccaggaggag tggtgtcttc ttagtgaggc tcagaggtgc
      121 ttgtaccgtg atgtgatgct agagaacctg gctctcatat cctcgctggg ttgttggtgt
      181 ggatcaaaag atgaggaggc accttgtaag cagagaattt ctgtacaaag agagtctcag
      241 agcaggactc ctagggcagg tgtttctcct aagaaggctc acccctgtga aatgtgtggc
      301 ctcatcttgg aggatgtttt tcactttgct gaccaccagg aaactcatca caagcagaag
      361 ctgaacagga gtggagcatg tggaaaaaac ttggatgaca ctgcatacct tcatcagcac
      421 cagaagcagc atattggaga gaaattctac agaaagagtg tcagagaagc atcgtttgta
      481 aagaaacgta agctcagggt gtcacaggag ccatttgtct tccgcgagtt tgggaaggac
      541 gttctgccca gttcaggatt gtgccaagaa gcagctgctg tagagaagac agacagtgaa
      601 actatgcatg gcccaccctt tcaggaggga aaaactaatt acagttgtgg aaaacgcaca
      661 aaagccttca gcaccaaaca ctcagttatt ccacaccaga aacttttcac tagagatgga
      721 tgttatgtgt gcagtgattg tggaaaatcc tttagcagat atgtcagctt cagtaatcat
      781 cagcgagatc acactgcaaa aggaccttat gattgtggag agtgtgggaa atcttatagt
      841 cgaaagagca gccttattca acatcagcga gtccacactg gaaagacagc ttatccctgt
      901 gaggagtgcg ggaaatcttt tagtcagaag ggcagcctta ttagccatca gcgtgttcac
      961 actggagaaa ggccttatga gtgtagagaa tatgggaaat cttttggtca aaagggtaac
     1021 ctcattcaac atcagcaagg tcacactgga gagagagctt atcactgtgg ggaatgtggg
     1081 aaatcttttc gtcagaagtt ctgctttatt aaccatcagc gtgttcacac tggagaaagg
     1141 ccttacaagt gtggagaatg tggaaaatct tttggtcaaa agggcaacct cgttcaacat
     1201 cagcgaggtc atactggaga aaggccctat gagtgcaagg aatgtgggaa atcatttagg
     1261 tacagatccc acctcactga acaccagaga cttcacactg gggaaagacc ttacaattgt
     1321 agggaatgtg ggaaattatt taacaggaag tatcatcttc tcgttcatga gagagttcac
     1381 actggagaaa ggccatatgc gtgtgaggta tgtgggaaat tatttggtaa taagaactgc
     1441 gtgactatac atcagaggat tcacactgga gaaaggccgt atgaatgcaa tgaatgtggg
     1501 aaatcatttc tttccagctc tgcgcttcat gttcataaaa gagttcattc tggacaaaag
     1561 ccttataagt gcagtgaatg tggaaaatcc tttgctgaat gttccagtct cattaaacac
     1621 aggagaattc acactggaga aaggccttat gaatgtacca aatgtggaaa aacatttcag
     1681 cgaagctcta ccctccttca tcatcagagt tcacacagga gaaaggcctt atagtaatga
     1741 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1801 gaccgttgtg cccagccgga ttactc
//
