LOCUS       sequence-282855-        2603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2197
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccggcgc gctggcaggt ctggccgcgg gcttgcaggt cccgcgggtc
       61 gcgcccagcc cagactcgga ctcggacaca gactcggagg acccgagtct ccggcgcagc
      121 gcgggcggct tgctccgctc gcaggtcatc cacagcggtc acttcatggt gtcgtcgccg
      181 cacagcgact cgctgccccg gcggcgcgac caggaggggt ccgtggggcc ctccgacttc
      241 gggccgcgca gtatcgaccc cacactcaca cgcctcttcg agtgcttgag cctggcctac
      301 agtggcaagc tggtgtctcc caagtggaag aatttcaaag gcctcaagct gctctgcaga
      361 gacaagatcc gcctgaacaa cgccatctgg agggcctggt atatccagta tgtgaagcgg
      421 aggaagagcc ccgtgtgtgg cttcgtgacc cccctgcagg ggcctgaggc tgatgcgcac
      481 cggaagccgg aggccgtggt cctggagggg aactactgga agcggcgcat cgaggtggtg
      541 atgcgggaat accacaagtg gcgcatctac tacaagaagc ggctccgtaa gcccagcagg
      601 gaagatgacc tcctggcccc taagcaggcg gaaggcaggt ggccgccgcc ggagcaatgg
      661 tgcaaacagc tcttctccag tgtggtcccc gtgctgctgg gggacccaga ggaggagccg
      721 ggtgggcggc agctcctgga cctcaattgc tttttgtccg acatctcaga cactctcttc
      781 accatgactc agtccggccc ttcgcccctg cagctgccgc ctgaggatgc ctacgtcggc
      841 aatgctgaca tgatccagcc ggacctgacg ccactgcagc caagcctgga tgacttcatg
      901 gacatctcag atttctttac caactcccgc ctcccacagc cgcccatgcc ttcaaacttc
      961 ccagagcccc ccagcttcag ccccgtggtt gactccctct tcagcagtgg gaccctgggc
     1021 ccagaggtgc ccccggcttc ctcggccatg acccacctct ctggacacag ccgtctgcag
     1081 gctcggaaca gctgccctgg ccccttggac tccagcgcct tcctgagttc tgatttcctc
     1141 cttcctgaag accccaagcc ccggctccca ccccctcctg tacccccacc tctgctgcat
     1201 taccctcccc ctgccaaggt gccaggcctg gagccctgcc ccccacctcc cttccctccc
     1261 atggcaccac ccactgcttt gctgcaggaa gagcctctct tctctcccag gtttcccttc
     1321 cccaccgtcc ctcctgcccc aggagtgtct ccgctgcctg ctcctgcagc cttcccaccc
     1381 accccacagt ctgtccccag cccagccccc acccccttcc ccatagagct tctacccttg
     1441 gggtattcgg agcctgcctt tgggccttgc ttctccatgc ccagaggcaa gccccccgcc
     1501 ccatccccta ggggacagaa agccagcccc cctaccttag cccctgccac tgccagtccc
     1561 cccaccactg cggggagcaa caacccctgc ctcacacagc tgctcacagc agctaagccg
     1621 gagcaagccc tggagccacc acttgtatcc agcaccctcc tccggtcccc agggtccccg
     1681 caggagacag tccctgaatt cccctgcaca ttccttcccc cgaccccggc ccctacaccg
     1741 ccccggccac ctccaggccc ggccacattg gccccttcca ggcccctgct tgtccccaaa
     1801 gcggagcggc tctcaccccc agcgcccagc ggcagtgaac ggcggctgtc aggggacctc
     1861 agctccatgc caggccctgg gactctgagc gtccgtgtct ctcccccgca acccatcctc
     1921 agccggggcc gtccagacag caacaagacc gagaaccggc gtatcacaca catctccgcg
     1981 gagcagaagc ggcgcttcaa catcaagctg gggtttgaca cccttcatgg gctcgtgagc
     2041 acactcagtg cccagcccag cctcaaggag cgtgcgggct tgcaggagga ggcccagcag
     2101 ctgcgggatg agattgagga gctcaatgcc gccattaacc tgtgccagca gcagctgccc
     2161 gccacagggg tacccatcac acaccagcgt tttgaccaga tgcgagacat gtttgatgac
     2221 tacgtccgaa cccgtacgct gcacaactgg aagttctggg tgttcagcat cctcatccgg
     2281 cctctgtttg agtccttcaa cgggatggtg tccacggcaa gtgtgcacac cctccgccag
     2341 acctcactgg cctggctgga ccagtactgc tctctgcccg ctctccggcc aactgtcctg
     2401 aactccctac gccagctggg cacatctacc agtatcctga ccgacccggg ccgcatccct
     2461 gagcaagcca cacgggcagt cacagagggc acccttggca aacctttata gtaatgaact
     2521 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2581 aactaatatc ggcacgctct ggt
//
