LOCUS       sequence-282864-        1628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2221
FEATURES             Location/Qualifiers
     source          1..1628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcccgg gcagcagccg ccgcctcaac cggcccccca gggccaaggg
       61 cagccgcctt cgcagccccc gcaggggcag ggcccgccgt ccggacccgg gcaaccggca
      121 cccgcggcga cccaggcggc gccgcaggca ccccccgccg ggcatcagat cgtgcacgtc
      181 cgcggggact cggagaccga cctggaggcg ctcttcaacg ccgtcatgaa ccccaagacg
      241 gccaacgtgc cccagaccgt gcccatgagg ctccggaagc tgcccgactc cttcttcaag
      301 ccgccggagc ccaaatccca ctcccgacag gccagtactg atgcaggcac tgcaggagcc
      361 ctgactccac agcatgttcg agctcattcc tctccagctt ctctgcagtt gggagctgtt
      421 tctcctggga cactgacccc cactggagta gtctctggcc cagcagctac acccacagct
      481 cagcatcttc gacagtcttc ttttgagata cctgatgatg tacctctgcc agcaggttgg
      541 gagatggcaa agacatcttc tggtcagaga tacttcttaa atcacatcga tcagacaaca
      601 acatggcagg accccaggaa ggccatgctg tcccagatga acgtcacagc ccccaccagt
      661 ccaccagtgc agcagaatat gatgaactcg gcttcaggtc ctcttcctga tggatgggaa
      721 caagccatga ctcaggatgg agaaatttac tatataaacc ataagaacaa gaccacctct
      781 tggctagacc caaggcttga ccctcgtttt gccatgaacc agagaatcag tcagagtgct
      841 ccagtgaaac agccaccacc cctggctccc cagagcccac agggaggcgt catgggtggc
      901 agcaactcca accagcagca acagatgcga ctgcagcaac tgcagatgga gaaggagagg
      961 ctgcggctga aacagcaaga actgcttcgg caggtgaggc cacaggcaat gcggaatatc
     1021 aatcccagca cagcaaattc tccaaaatgt caggagttag ccctgcgtag ccagttacca
     1081 acactggagc aggatggtgg gactcaaaat ccagtgtctt ctcccgggat gtctcaggaa
     1141 ttgagaacaa tgacgaccaa tagctcagat cctttcctta acagtggcac ctatcactct
     1201 cgagatgaga gtacagacag tggactaagc atgagcagct acagtgtccc tcgaacccca
     1261 gatgacttcc tgaacagtgt ggatgagatg gatacaggtg atactatcaa ccaaagcacc
     1321 ctgccctcac agcagaaccg tttcccagac taccttgaag ccattcctgg gacaaatgtg
     1381 gaccttggaa cactggaagg agatggaatg aacatagaag gagaggagct gatgccaagt
     1441 ctgcaggaag ctttgagttc tgacatcctt aatgacatgg agtctgtttt ggctgccacc
     1501 aagctagata aagaaagctt tcttacatgg ttatagtaat gaactagtcc gtaacttgaa
     1561 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatacgga cggttgtcca
     1621 acctttag
//
