LOCUS       sequence-282888-        1859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2318
FEATURES             Location/Qualifiers
     source          1..1859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaactctt aacattcagg gatgtggcca tagaattctc tccagaagag
       61 tggaaatgcc tggaccctgc ccagcagaat ttgtatagag atgtgatgtt ggagaactac
      121 aggaacctga tctccctggg tgttgctatc tctaacccag acctggtcat ctatctggag
      181 caaagaaagg agccctacaa agtgaagata catgagacag tagccaaaca cccagctgtg
      241 tgttctcatt tcacccaaga ctttttgcca gtgcagggga tagaagattc gttccacaaa
      301 cttatattaa gaagatatga gaaatgtgga catgagaatt tagaattaag aaaaagctgt
      361 aaaaggaagg tgcagaaagg aggttataat gaatttaatc aatgcttgtc aactatccag
      421 agcaaaatat ttcagtgtaa tgtacatgtc aaagtattta gtacattttc aaattcaaac
      481 caacgtagga taagacatac tggagagaaa cactttaaag aatgtggcaa gtcatttcag
      541 aagttctcag acctaactca acatcaagga attcatgctg gagagaaacc ctacacatgt
      601 gaagaatgtg gcaaagactt taaatggtat ttaatcttta atgaatatga gataattcat
      661 actggagaga aacccttcac atgtgaagaa tgtggcaaca tctttaccac atcctcaaac
      721 tttgctaaac ataaagttca tactggagag aaatcttaca aatatgaaga atgtggcaaa
      781 gcctttaata ggtcctcaac ccttactaag cataagagaa ttcatgctga agagaaaccc
      841 ttcacatgtg aagaatgtgg caaaatcatt acctcatcct caaatgttgc caaacataag
      901 aaaattcata ccggagagaa actctacaaa tgtcaggaat gtggcaaagt ctttaatagg
      961 tccacaaccc ttactaaaca taacagaatt catactggag agaaacccta cacatgtgaa
     1021 gaatgtggca aagcctttag taggtcctca gttctgaatg aacataagag aattcatact
     1081 ggagagaagc cctacaaatg tgaacaatgt ggcaaagcct ttagacaatc cgcaaccctt
     1141 aataaacata agagtattca tactggagag aaaccctaca catgtgaaga atgtggaaaa
     1201 gcctttagtc ggttcacaac ccttaatgaa cataagagaa ttcatactgg agagaggccc
     1261 cacaaatgtg aagagtgtgg caaagccttt ggatggtcca cagacctgaa taaacataag
     1321 ataattcata ctggagagaa accttacaaa tgtgaagagt gtggcaaagc ctttggatgg
     1381 tctgcatacc tgagtaaaca taagaaaatt catactggag agaaacctta tagatgtgaa
     1441 gagtgtggca aagccttttt atgttccaga gccctgaata aacataagac aattcatact
     1501 ggagagaaac cttacgaatg tgaagagtgt ggcaaagcct ttggatggtc cacatacctg
     1561 agtaaacata agaaaattca tactggagag aaaccttata gatgtgaaga gtgtggcaaa
     1621 gcatttagac gttccagagt cctgaataaa tataagacaa ttcatactgg agataaaacc
     1681 cccaaatgta aaggatgtgg caaagccttt aagtggtcct cataccttaa tcaacataat
     1741 aaaatttata ctggagagaa actctagtaa tgaactagtc cgtaacttga aagtatttcg
     1801 atttcttggc tttatatatc ttgtggaaag gacgactcgc gatcacaggg atcatactt
//
