LOCUS       sequence-282906-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2581
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccggag gtatgatgag ctgccgcact acccaggcat cgtggatggc
       61 cccgcagccc tggctagctt cccagagaca gtgcccgcag taccagggcc ctatggcccg
      121 caccggcctc cccagcccct gcccccaggc ttggacagcg acggcctgaa gagggagaag
      181 gatgagatct atggacaccc gctcttcccc ctcttggccc tggtctttga gaaatgtgaa
      241 ctggctacat gctctccccg tgacggggcc ggagctgggc tggggacacc ccctggaggt
      301 gacgtctgct cctctgattc cttcaacgag gacatcgctg cctttgccaa gcaggttcgc
      361 tctgagaggc ccctcttctc ctccaaccca gaactggaca atctgatgat ccaggccatc
      421 caggtgctgc ggttccacct gctggagctg gagaaggtcc acgacctgtg cgacaacttc
      481 tgtcaccgct acatcacctg cctcaaggga aagatgccca tcgacctggt catcgaggat
      541 cgggacggcg gctgcaggga ggacttcgag gactacccag cctcctgccc cagcctccca
      601 gaccagaata atatgtggat tcgagaccat gaggatagtg ggtctgtaca tttggggacc
      661 ccaggtccat ccagtggggg cctggcctcc cagagtgggg acaactccag tgaccaagga
      721 gacgggctgg acaccagcgt ggcctctccc agttctggtg gagaagatga ggacttggac
      781 caggagcgac ggcgaaacaa gaagaggggg atcttcccca aggtggccac caacatcatg
      841 cgagcctggt tgttccagca cctctcgcac ccgtacccct cggaggagca gaagaaacag
      901 ctggcgcagg acacggggct caccatcctg caagtcaaca actggttcat taacgcccgg
      961 agacgcatcg tgcaacctat gatcgatcaa tccaaccgca cagggcaggg tgcagccttc
     1021 agcccagagg gccagcccat cgggggctat accgagacgc agccacacgt ggccgtccgg
     1081 cctccgggat cagtggggat gagtttgaac ttggaaggag aatggcatta tctatagtaa
     1141 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1201 gacgacacgc cttcaatacg ggcgcgcat
//
