LOCUS       sequence-282909-        2063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2615
FEATURES             Location/Qualifiers
     source          1..2063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctccag gcttccgacg gcctggtcct gtgaaccagt gacctttgaa
       61 gatgtaacac tgggttttac cccggaagag tggggactgc tggacctcaa acagaagtcc
      121 ctgtacaggg aagtgatgct ggagaactac aggaacctgg tctcagtgga acatcagctt
      181 tccaaaccag atgtggtatc tcagttagag gaggcagaag atttctggcc agtggagaga
      241 ggaattcctc aagacaccat tccagagtat cctgagctcc agctggaccc taaattggat
      301 cctcttcctg ctgagagtcc cctaatgaac attgaggttg ttgaggtcct cacactgaac
      361 caggaggtgg ctggtccccg gaatgcccag atccaggccc tatatgctga agatggaagc
      421 ctgagtgcag atgcccccag tgagcaggtc caacagcagg gcaagcatcc aggtgaccct
      481 gaggccgcgc gccagaggtt ccggcagttc cgttataagg acatgacagg tccccgggag
      541 gccctggacc agctccgaga gctgtgtcac cagtggctac agcctaaggc acgctccaag
      601 gagcagatcc tggagctgct ggtgctggag cagttcctag gtgcactgcc tgtgaagctc
      661 cggacatggg tggaatcgca gcacccagag aactgccaag aggtggtggc cctggtagag
      721 ggtgtgacct ggatgtctga ggaggaagta cttcctgcag gacaacctgc cgagggcacc
      781 acctgctgcc tcgaggtcac tgcccagcag gaggagaagc aggaggatgc agccatctgc
      841 ccagtgacag tgctccctga ggagccagtg accttccagg atgtggctgt ggacttcagc
      901 cgggaggagt gggggctgct gggcccgaca cagaggaccg agtaccgcga tgtgatgctg
      961 gagacctttg ggcacctggt ctctgtgggg tgggagacta cactggaaaa taaagagtta
     1021 gctccaaatt ctgacattcc tgaggaagaa ccagccccca gcctgaaagt acaagaatcc
     1081 tcaagggatt gtgccttgtc ctctacatta gaagatacct tgcagggtgg ggtccaggaa
     1141 gtccaagaca cagtgttgaa gcagatggag tctgctcagg aaaaagacct tcctcagaag
     1201 aagcactttg acaaccgtga gtcccaggca aacagtggtg ctcttgacac aaaccaagtt
     1261 tcgctccaga aaattgacaa ccctgagtcc caggcaaaca gtggcgctct tgacacaaac
     1321 caagttttgc tccacaaaat tcctcctaga aaacgattgc gcaaacgtga ctcacaagtt
     1381 aaaagtatga aacataattc acgtgtaaaa attcatcaga agagctgtga aaggcaaaag
     1441 gccaaggaag gcaatggttg taggaaaacc ttcagtcgga gtactaaaca gattacgttt
     1501 ataagaattc acaaggggag ccaagtttgc cgatgcagtg aatgtggtaa aatattccgg
     1561 aacccaagat acttttctgt gcataagaaa atccataccg gagagaggcc ctatgtgtgt
     1621 caagactgtg ggaaaggatt tgttcagagc tcttccctca cacagcatca gagagttcat
     1681 tctggagaga gaccatttga atgtcaggag tgtgggagga ccttcaatga tcgctcagcc
     1741 atctcccagc acctgaggac tcacactggc gctaagccct acaagtgtca ggactgtgga
     1801 aaagccttcc gccagagctc ccacctcatc agacatcaga ggactcacac cggggagcgc
     1861 ccatatgcat gcaacaaatg tggaaaggcc ttcacccaga gctcacacct tattgggcac
     1921 cagagaaccc acaataggac aaagcgaaag aagaaacagc ctacctcata gtaatgaact
     1981 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2041 tgttctatcc acctcgtagc tac
//
