LOCUS       sequence-282924-        1808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2682
FEATURES             Location/Qualifiers
     source          1..1808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacgttt aacaaaaaga cgacaggcgg atacaaaagc tatccagcat
       61 ctttgggcag ccattgagat tatacggaac cagaagcaga ttgccaacat tgaccgtatt
      121 acaaagtata tgtctcgagt ccacggtatg caccctaaag agaccacccg tcagctgagc
      181 ttagctgtga aagatggtct tattgtcgaa actctaacag tgggctgcaa aggttcaaaa
      241 gctggtattg aacaagaagg atattggttg ccaggagatg agattgactg ggaaacagaa
      301 aatcatgact ggtattgttt tgaatgccat ttgcctggag aggtgttgat atgtgacctg
      361 tgttttcgtg tgtatcattc caagtgtttg tctgatgagt tcaggcttag agacagcagt
      421 agtccctggc agtgcccagt ttgcaggagc attaagaaga agaatacaaa caaacaggag
      481 atgggcacat acctcagatt cattgtctcc cgcatgaagg agagggctat agatcttaat
      541 aaaaagggga aggacaataa acacccgatg tacaggaggc tggtgcactc agctgtggac
      601 gttcccacca ttcaagagaa agtgaatgaa gggaaatacc gaagttatga agagttcaaa
      661 gctgatgccc aattgcttct ccacaatacc gtgattttct atggagcaga cagtgagcaa
      721 gctgacattg cgaggatgct atataaagac acatgtcatg agctggatga actgcagctt
      781 tgcaagaatt gcttttactt gtcaaatgct cgtcctgaca actggttctg ttatccttgt
      841 atacctaatc atgagctggt ttgggctaaa atgaaaggtt ttgggttttg gccagccaaa
      901 gtcatgcaga aagaagacaa tcaagtcgac gttcgcttct ttggccacca ccaccagagg
      961 gcctggattc cttctgaaaa cattcaagat atcacagtca acattcatcg gctgcacgtg
     1021 aagcgcagta tgggttggaa aaaggcctgt gatgagctgg agctgcatca gcgtttccta
     1081 cgagaaggga gattttggaa atctaagaat gaggaccgag gtgaggaaga ggcagaatcc
     1141 agtatctcct ccaccagtaa tgagcagcta aaggtcactc aagaaccaag agcaaagaaa
     1201 ggacgacgta atcaaagtgt ggagcccaaa aaggaagaac cagagcctga aacagaagca
     1261 gtaagttcta gccaggaaat acccacgatg cctcagccca tcgaaaaagt ctccgtgtca
     1321 actcagacaa agaagttaag tgcctcttca ccaagaatgc tgcatcggag cacccagacc
     1381 acaaacgacg gcgtgtgtca gagcatgtgc catgacaaat acaccaagat cttcaatgac
     1441 ttcaaagacc ggatgaagtc ggaccacaag cgggagacag agcgtgttgt ccgagaagct
     1501 ctggagaagc tgcgttctga aatggaagaa gaaaagagac aagctgtaaa taaagctgta
     1561 gccaacatgc agggtgagat ggacagaaaa tgtaagcaag taaaggaaaa gtgtaaggaa
     1621 gaatttgtag aagaaatcaa gaagctggca acacagcaca agcaactgat ttctcagacc
     1681 aagaagaagc agtgggtaaa taccagtctt ttttagtaat gaactagtcc gtaacttgaa
     1741 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaccttta tgtagcgatc
     1801 cgagcagc
//
