LOCUS       sequence-282936-        1961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2730
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagtgga ggctgcagag gcccggtccc cagcccccgg ctacaagcgc
       61 tcgggccgcc gctacaagtg cctgtcctgt accaagacat ttccaaacgc gcccagggca
      121 gcgcgccacg ctgccacaca tgggccggca gactgctctg aagaggtggc cgaggtgaag
      181 ccaaagccag agacagaagc taaggcagag gaagccagtg gggagaaggt gtcaggctcc
      241 gcggccaagc ctaggcccta tgcgtgtccg ctatgcccca aggcctacaa gacggcaccc
      301 gagctgcgca gccacgggcg cagccacacg ggggagaagc cctttccgtg ccccgagtgc
      361 ggccgccgct tcatgcagcc cgtgtgcctg cgcgtgcacc tggcctcgca cgctggcgaa
      421 ctgcccttcc gctgtgcgca ctgcccgaag gcctatggcg cgctctccaa gctcaagatc
      481 caccagcgtg gccacacagg cgagcggcct tacgcctgcg ccgactgcgg caagagcttt
      541 gctgaccctt cagtgttccg caagcaccgg cgtactcacg ctggcctgcg gccctacagc
      601 tgtgagcgtt gcggcaaagc ctatgcggag ctcaaggacc tccgcaacca tgagcggtcc
      661 cacaccggcg agcgcccctt cctctgctcc gagtgcggga agagcttctc ccgctcatcc
      721 tcgctcacgt gccaccagcg catccacgcg gcacagaagc cctaccgctg cccggcctgc
      781 ggcaagggct tcacgcagct cagttcctac cagagccacg agcgcacgca ctcgggggag
      841 aagcccttcc tgtgcccgcg ctgcggccgc atgttctccg acccctcgag cttccgtcgc
      901 caccagcgcg cccatgaagg ggtgaagcca taccactgcg agaagtgcgg caaggacttc
      961 cggcagccgg cggacctggc catgcaccgg cgtgtgcaca caggcgaccg gccgttcaag
     1021 tgcctgcaat gtgacaagac gttcgtggcg tcctgggacc tcaagcggca cgcgctggtg
     1081 cactctggcc agcggccctt ccgctgtgag gagtgcgggc gagccttcgc cgagcgtgcc
     1141 agcctcacga agcatagccg ggtgcactcg ggggagcgcc ccttccactg taacgcatgt
     1201 gggaaatcct ttgtggtgtc gtcgagcctg aggaagcacg agcggaccca tcgaagcagt
     1261 gaggccgcgg gtgtgccccc tgcacaggag ctggtggtgg ggttggcgct gcctgtgggc
     1321 gtggcaggtg agagttcagc cgccccggca gcaggggcgg ggctggggga ccctccagca
     1381 gggctgctag ggctgccccc ggagtcaggt ggtgtgatgg ccacacagtg gcaggtggtg
     1441 ggcatgacgg tggagcatgt ggaatgccaa gatgctggtg tccgggaggc tcctggtccc
     1501 ttggaagggg caggcgaggc ggggggtgag gaggctgacg agaagccccc ccagtttgtg
     1561 tgccgagagt gcaaggagac cttctccaca atgacgctgc tgcgtcggca cgagcgctca
     1621 cacccggagc tccggccctt cccctgcacc cagtgcggca agagcttctc tgaccgggct
     1681 gggctgcgca aacacagccg cactcacagc tcagtgcgcc cctacacctg cccccattgt
     1741 cccaaggcct tcttgagtgc cagcgacttg cgcaagcatg aacgcaccca ccctgtgccc
     1801 atggggaccc ccacacccct ggagcccctg gtggctttgc taggaatgcc tgaagagggg
     1861 ccggcctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1921 tcttgtggaa aggacgactc ttaccacaaa tgggataccg a
//
