LOCUS       sequence-28294-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Meylan E, Dooley AL, Feldser DM, Shen L, Turk E, Ouyang C, Jacks T
  TITLE     Requirement for NF-kappaB signalling in a mouse model of lung 
            adenocarcinoma.
  JOURNAL   Nature. 2009 Nov 5;462(7269):104-7. doi: 10.1038/nature08462. Epub 
            2009 Oct 21.
  PUBMED    19847165
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08462"; journalName: "Nature"; date: "2009-11-5- 5"; 
            volume: "462"; issue: "7269"; pages: "104-7"
COMMENT     Sequence Label: pSicoR (Puro) NEMO shRNA2
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        160..659
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(195..218)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     592..611
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(703..722)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cnaaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt ttggacaagg
       61 cctctgtgaa attcaagaga tttcacagag gccttgtcct tttttctcga gtactaggat
      121 ccattaggcg gccgaattcg tcgacctcga aattctaccg ggtaggggag gcgcttttcc
      181 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
      241 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
      301 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
      361 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
      421 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
      481 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
      541 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
      601 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
      661 cctgcatcca tctagatctc gatcgagcag cttaagctta ccatgaccga gtacaagccc
      721 acggtgcgcc tcgccacccg cga
//
