LOCUS       sequence-282945-        6344 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2745
FEATURES             Location/Qualifiers
     source          1..6344
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgagctcctc cccctcccgc agtgccgccg acatctacat ccgggagtat
       61 ttccacagcc acgtcagcgg cggccacccc gaggccactc ctctccgtgt gatgtacacg
      121 gaccggccgc tgagccagac ggacccagtc acgctgcagt actgttgcct gaccgacgac
      181 cgccaggctt tccgcccgcc cacacgggca gagctggcgc ggcaccgcgt ggtggtcacc
      241 accacctccc aggcccgtga gctcagggtg ccggtcggct tcttctccca cattctcatc
      301 gatgaggcgg cccagatgct ggagtgcgag gccctcaccc cgctggccta tgcctcgcac
      361 ggcacccgcc tcgtgctggc gggcgaccac atgcaggtca caccccggct gttcagtgtg
      421 gccagggccc gggcggccga gcacacgctg ctgcaccgcc tcttcctgtg ctaccagcag
      481 gagactcacg aggtggcgcg gcagagccgc ctggtcttcc acgagaacta ccgctgcacg
      541 gacgccattg tcagcttcat ctcgcggcac ttctacgtgg ccaagggcaa ccccatccac
      601 gccaggggca aggttccgcc ccacccccgg cactacccgc tcatgttctg ccacgtggcg
      661 ggcagcccag accgggacat gtccatggcg tcctggctga atctggctga gattgcgcag
      721 gtcgtcgaga aggtgcagga ggcctacaac acctggccca gctgctgggg cggccgcgag
      781 cagaggtgca tctgtgtcgt ttcccacggt gcccaggtca gtgcactgag gcaggagctg
      841 aggaggcggg acctaggcca ggtgtctgtc ggcagttttg agatcctgcc agggcggcag
      901 ttccgggtcg tggtgctcag cactgtgcac acctgccaga gcctgctcag ccctggggca
      961 ctggcccctg agttcttcac cgacgcccgc gtgctcaaca ccgtcctgac ccgcgcccag
     1021 tcccagctgg tggtagtggg ggacgccgtg gccctctgct ccttcggggc ctgcggcaag
     1081 ctctgggaga gcttcatccg tgagtgcgtg gagcggcaca gtgtctgccc cgagggcctg
     1141 tccatggagc aggtcgagca gggtgtggcg cagagacggc gctggcctcc ccgaggcaca
     1201 caggctgggg cagcggggaa ctgggaggct gccccagagc cagtagggga cctggccgag
     1261 gagcaggcgg ctgtggtgac ggccatggtg aaggcagagc cgggagatga ggctctgagc
     1321 ccagcatccc gtgacatcac ggcaaccaca gcgcagacgg aggctgcggc agcaccagca
     1381 ggagacgcag tgaaggaaga cgtggtgccc ggggcctgtg cggcaggagc ggctgctgca
     1441 gcgggcgtgg agtccacgga ggctgaggat gcagaggctg acttctggcc gtgggacggg
     1501 gagctcaacg ctgacgacgc catcctacgg gagcttctgg acgagagcca gaaggtgatg
     1561 gtgaccgtcg gggaggacgg gctgctggac actgtcgcca ggcccgagtc cctgcagcag
     1621 gcccggctgt acgagaacct gcccccggct gcgctacgga agctgctgca cgcggagccc
     1681 gagcggtacc gccactgctc tttcgtgcca gagaccttcg agcgggcgtc agccatcccg
     1741 ctggacgatg cctcctcggg ccccatccag gtcaggggcc gcctggactg tgggatggcc
     1801 ttcgccgggg atgaggtgct ggtgcagctc ctttcgggag acaaggcgcc cgaggggcgg
     1861 cttcggggcc gcgtgctggg cgtgctgaag aggaagaggc acgagctggc gtttgtgtgc
     1921 cgcatggaca cgtgggaccc gcgcatcatg gtccccatca atggctccgt gaccaagatc
     1981 ttcgtggccg agctgaagga cccatcgcag gtccccatct acagcctccg gaagggccgg
     2041 ctgcagcgtg tggggcttga gaggctcacc gccgaggccc ggcacagccg gctcttctgg
     2101 gtccaaattg tcctgtggcg gcaaggcttc tactacccgc tgggcatcgt ccgggaggtg
     2161 ctgcctgagg ccagcacctg ggagcagggc ctccgcatcc tcggcctgga gtacagcttg
     2221 agggtgcccc cgtcggacca ggccaccatc accaaggtgc tgcagaaata ccacacggag
     2281 cttggccggg ttgccggccg ccgagaggac tgccgcgcct tcttgacctt cactgtggac
     2341 ccccagggcg cctgcaacct cgatgatgcc ctcagtgtcc gagacctggg tcccaggtgc
     2401 gaggtggctg tgcacatcac tgatgtggcc agcttcgtgc ccagggacgg ggtgctggac
     2461 gtggaggcgc gaaggcaggg cgctgcgttc tatgcccccg gcagggagcc agtgcccatg
     2521 ctgccggcca gcctctgcca ggacgtcctc agcctcctgc ctggccggga ccgcctggcc
     2581 atctccctgt tcctcaccat ggagaaggcc agtggccagc tgaagagcct gcgctttgca
     2641 ccctccgtgg tccagtctga ccgccagctg tcctacgagg aggcggagga ggtgatcagg
     2701 cagcacccgg gtgccggccg tgagctgccg gcccgcctgg actccgtgga cgcctgcgtc
     2761 gtggccgcgt gctacttctc tcggctgctg cgccggcacc gcctgcggtc cgactgcttc
     2821 tatgagcagc cggacgagga cggcaccctg ggcttccgcg cggcccacat catggtgaag
     2881 gagtacatga ttcagtttaa taggctcgtg gctgagttcc tggtgggcag cgagtgcacg
     2941 cggacggtca cgcctctgcg gtggcagcca gcaccccgca gccagcagct caaggccctg
     3001 tgtgagaagc atggggaccg ggtgcccctg tcactgcacc tcggccacca cctgcacggc
     3061 ggcgggggca gtccccccga cacgcggctg cacctcctgg cctccctctg gaagcaggtc
     3121 cagtttgctg cccgcaccca ggactacgaa cagatggtgg acttggtcac cacggacgac
     3181 atgcacccat tcctggctcc tgcaggccgc gacctccgca aggccctgga gcgctcggcg
     3241 ttcggccgct gcgcccgggg ccaccagcag cagggcggcc actactcgct gcaggtggac
     3301 tggtacacgt gggccacctc gcccatccgc aggtacctgg acgtggtgtt gcagcggcag
     3361 atcctgctgg cgctgggcca tgggggctct gcctactctg ccagggacat cgatgggctc
     3421 tgccaggcct tcagcctcca gcacgcactt gcccagagct atcagcggcg ggcgcgcagc
     3481 ctgcacctgg ccgtgcagct caaggcccag cctctggaca agctgggctt cgtggtggac
     3541 gtggaggcgg gctcccgctg cttccggctg ctcttcccca gcaaccggga gacgctgcct
     3601 gacccctgcc ccgtccccta cggctccctg cagctggccg agcaccccca cgccctggca
     3661 ggccggccgg gcctgcggct cctgtggcgg cgccgtgtct actcagcgca gggatccagc
     3721 ccgcccctgc cactgcctgg cactgtgccg gacccacaca ccctggccgt ggagacggcc
     3781 ctgtggaagc agctgctgga gctggtggag ctgcagcgct ggccggaggc ggctgctctc
     3841 atccaggaga agggcgaggc gtcccagcgg cgggagctgg tgcaggtgca gcggagccac
     3901 tgtggccatt tcctggaggt ggcccgggag ctgggcagtg gggacaccct gcaggtgcag
     3961 ctcggcacca gcctgcagca cggcttcctg gtaccgagcc ctcagctctg gacggtggca
     4021 ccgggcttca gcctctgcct ggagcacgtg gagcggcccg gagactgctt ctcaggccgt
     4081 gtgtaccggg ccccgaggga ccggtaccgc gacgtggatg agtacgcctg cgtgtgggaa
     4141 ccattctgcg ccctggagtc ggccaccggc gcggttgccg agaatgactc cgtcacactt
     4201 cagcacctga gtgtctcctg ggaggcgtca cggacgccgc aggggcagct gcagggcgcc
     4261 ttccgcctgg aggccgcctt cctcgaggag aactgtgccg acatcaactt cagctgctgc
     4321 tacctctgca tccggctcga ggggctgccg gctcccacgg ccagcccacg ccctgggccc
     4381 agcagcctcg gccctggcct gaatgttgac cccggcacgt atacctgggt ggcccacggg
     4441 cagacgcagg actgggacca ggagcgccgg gcagaccggc aggaggctcc cagacgggtg
     4501 cacctcttcg tccaccacat gggcatggag aaggttccgg aagaggtgct gaggccgggc
     4561 accctgttca ccgttgagct gctgcccaag cagcttcctg atctccgcaa ggaggaagcc
     4621 gtgcgtggac tagaggaggc gtccccgctg gtcaccagca tcgcactggg ccggcctgtc
     4681 ccgcagcccc tctgcagagt gatccccagc aggttcctgg agcggcagac ctacaacatc
     4741 cccggaggcc gccacaagct gaaccccagc cagaacgtgg cggtcaggga ggctctggag
     4801 aagcctttca cggtcattca gggcccacca ggtacaggga agacgatcgt gggcctccac
     4861 atcgtattct ggtttcataa atcaaaccag gagcaggtgc agcccggagg ccccccccgt
     4921 ggggagaagc ggctgggggg tccctgcatc ttgtactgcg gcccctccaa caagtcggtg
     4981 gatgtcctgg caggactgct cctgagaagg atggagctga agcccctccg tgtgtacagt
     5041 gagcaggctg aggccagcga gttcccagtg ccgcgtgtgg gcagcaggaa gctgctcagg
     5101 aagagccccc gggaggggag gccgaaccag agcctcagga gcatcaccct gcaccaccgg
     5161 atccggcagg cccccaaccc ttactcgtcg gaaatcaagg cctttgacac ccggctgcag
     5221 agaggggagc tcttctccag ggaggacctg gtctggtaca agaaggtctt gtgggaggct
     5281 cggaagttcg agctggaccg gcatgaggtc atcctctgca cctgctcctg tgcagcctct
     5341 gccagcctca aaatcctgga cgtgaggcag atccttgttg acgaggcagg catggccacg
     5401 gaacctgaaa ccctcatccc cctggtgcag ttcccacagg ccgagaaggt ggttcttctc
     5461 ggagaccaca agcagctgcg gcctgtggtc aagaatgagc ggctgcaaaa cctgggtctg
     5521 gaccggtctc tgttcgagcg gtaccacgag gacgcacata tgctggacac tcagtaccgc
     5581 atgcatgagg gcatctgtgc cttcccctct gtggcgttct acaagagcaa gctgaagacg
     5641 tggcagggcc tgaggaggcc gcccagtgtc ctgggccacg ctggcaagga gagctgccct
     5701 gtcatctttg gccacgtgca gggccacgag cggagcctgc tggtgtccac ggacgaaggg
     5761 aatgagaact ccaaggccaa cctggaggag gtggctgagg tggtccgtat caccaagcag
     5821 ctgaccctgg ggaggaccgt agagccccag gacatcgccg tcctcacgcc ctacaacgcg
     5881 caggcctctg agatcagcaa ggcccttcgg cgagagggca tcgccggggt ggccgtgtcc
     5941 tccatcacca agagccaggg gagcgagtgg cgctatgtgc tggtgagcac cgtccgcacc
     6001 tgtgccaaga gcgacctgga ccagcggccc accaagagct ggctcaagaa gtttctgggc
     6061 ttcgttgtgg accccaacca agtgaatgtg gctgtcacgc gggcccagga ggggctctgc
     6121 ctgatcggag accacctcct tctgcgctgc tgccccctct ggcgtagcct cctggacttc
     6181 tgcgaggctc agcagaccct cgtgcctgcc ggccaggtgc gcgtctgcag gaggccaact
     6241 atgccttcct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     6301 atatcttgtg gaaaggacga tcaggtaatc gtagacatat aact
//
