LOCUS       sequence-282954-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2757
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaggctc caatggcttt gggatcgact ccattctctc ccaccgcgcg
       61 ggcagccccg cccttcccaa gggggacccc ttgctcgggg actgccgttc gcccctggag
      121 ctgagtccac gctcagagag cagcagcgac tgctcttcgc cagcctctcc aggaagggac
      181 tgtttggaga cggggacccc acggcctggc ggggcatccg gcccaggttt ggactcccac
      241 ctgcagcccg ggcagctctc agccccggcc cagtcgcgca ccgtcacctc ctcctttctg
      301 atcagggaca tccttgccga ctgcaaacca ctcgcggcct gtgcacccta ctctagcagc
      361 gggcagccgg cagcccctga gcctgggggc cgccttgcgg ccaaggccgc ggaggacttt
      421 agagacaagc tggacaaaag tggcagcaac gcctcatcgg actctgagta taaagtgaag
      481 gaggagggcg accgcgagat ctccagctcc agggacagtc ccccggtgcg cctgaaaaag
      541 ccacgcaagg cgcgcacggc cttcaccgac catcagctgg cgcagctgga gcgcagcttc
      601 gagcggcaga agtacctgag cgtgcaggac cgcatggagc tcgccgcctc gctcaacctc
      661 accgacacgc aggtcaagac ctggtaccag aaccgcagga ctaaatggaa gcgacagacg
      721 gccgtcgggt tggagctgct ggcggaggca ggcaattact cagcgctcca gcggatgttc
      781 ccgtcgcctt atttctaccc gcagagtctg gtttccaacc tggaccccgg cgcggcgctc
      841 tacctgtacc gcggccccag cgcgccgccg cctgctctcc agagacctct ggtgccccgc
      901 atcctcatcc acggactcca gggcgccagc gagccgcccc cgccgctgcc ccccctggcc
      961 ggcgtcctcc cacgcgccgc gcagcctcgg tagtaatgaa ctagtccgta acttgaaagt
     1021 atttcgattt cttggcttta tatatcttgt ggaaaggacg aagggcacta tcacgggaag
     1081 acact
//
