LOCUS       sequence-282957-        2117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2758
FEATURES             Location/Qualifiers
     source          1..2117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctcagt ggctttagag gatgtggctg tgaacttcac ccgagaagag
       61 tgggctttgc tgggtccttg tcagaagaat ctctacaaag atgtgatgca ggaaaccatc
      121 aggaacctgg attgtgtagt aatgaaatgg aaagaccaga acattgaaga tcaatataga
      181 tatcccagga aaaatctaag atgtcgtatg ttagagagat ttgttgaaag taaagatgga
      241 actcaatgtg gagaaacatc tagccagatt caagatagta ttgtgaccaa gaacactctt
      301 cctggagtag gtccttgtga aagcagtatg agaggagaaa aagtcatggg tcattcatcc
      361 cttaattgtt acatcagagt tggtgctggg cacaaaccac atgagtatca tgaatgtgga
      421 gagaagccag atacgcataa acaacgtggg aaagccttca gttaccacaa ctcatttcaa
      481 acacatgaga ggcttcacac tggaaagaaa ccatatgatt gtaaagaatg tgggaagtcc
      541 ttcagttctt tgggaaacct tcaaagacac atggcagtgc agcgtggaga tggaccttat
      601 aaatgtaagt tgtgtgggaa agcgtttttt tggcccagtt tattacatat gcatgaaaga
      661 acgcacactg gagagaaacc atatgaatgt aagcagtgtt ctaaagcctt ttctttttac
      721 agttcctatc taagacatga aagaacacat actggggaga aaccgtatga atgtaaacag
      781 tgttctaaag cctttccttt ttacagttcc tatctaagac atgaaagaac acatactggg
      841 gagaaaccat ataaatgtaa gcagtgttct aaagccttcc ctgattccag ttcttgtcta
      901 atacatgaaa gaactcacac tggagagaaa ccctatacat gtaaacaatg tgggaaagcc
      961 ttcagtgttt ccggttccct tcaaagacat gaaaccactc acagtgcaga gaaaccctat
     1021 gcatgtcagc aatgtgggaa agcgtttcat catctgggaa gctttcaaag acacatgata
     1081 aggcacactg gaaatggacc tcataaatgt aagatatgtg ggaaaggctt tgattgtcct
     1141 agttcactgc aaagtcatga aagaactcac actggagaga aaccctatga atgcaagcag
     1201 tgtgggaaag cattatctca tcgctcaagc tttcgaagtc atatgataat gcacactgga
     1261 gatggacctc ataaatgcaa ggtatgtggg aaagcctttg tttatcccag tgtatttcaa
     1321 agacatgaaa ggactcacac tgcagagaaa ccctataaat gtaaacaatg tggcaaagcc
     1381 taccgtattt ccagttccct tcgaaggcat gaaacaactc atactggaga gaaaccctat
     1441 aaatgcaaac ttgggaaagc ctgtattgat ttctgttcct ttcaaaatca caaaacaact
     1501 cacactggag agaagccata tgagtgtaag gaatgtggga aagcattcag tcgtttcaga
     1561 tacctttctc gacataaaag gactcacaca ggagagaaac cttatgagtg taaaacatgt
     1621 aggaaagcct tcggtcatta tgataactta aaggtacatg aaagaattca ctctggagag
     1681 aagccgtatg aatgtaagga atgtgggaaa gcattctctt ggctcacttg ctttctacga
     1741 catgaaagaa ttcacatgag agagaaatcc tatgaatgtc cacaatgtgg taaagccttc
     1801 actcattccc gttttcttca aggacatgaa aaaactcata ctggagagaa cccgtatgaa
     1861 tgtaaggaat gtgggaaagc atttgcttct ctcagttcct tgcatagaca taaaaagact
     1921 cactggaaaa aaactcacac tggagagaac ccatatgaat gtaaggaatg tgggaaagca
     1981 tttgcttctc tcagttcctt gcatagacat aaaaagactc actagtaatg aactagtccg
     2041 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacaccact
     2101 atttagcctg ggaccac
//
