LOCUS       sequence-282960-        1718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2761
FEATURES             Location/Qualifiers
     source          1..1718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctagaga attaagtgaa agcacagccc tggatgccca gtctacagaa
       61 gaccagatgg agcttctggt cataaaggtg gaggaagaag aagccggttt tcccagtagc
      121 ccagatctgg gttctgaggg ctcccgcgag cgcttccgag gcttccgcta cccggaggct
      181 gcaggccccc gcgaggcgct gagtcggctc cgagagctct gccgacagtg gctgcagcct
      241 gagatgcaca gcaaggagca gatcctggag ctgctggtgc tggagcagtt cctgaccatc
      301 ctgccgggga atctgcagag ctgggtgcgg gagcagcatc cagagagcgg ggaggaggtg
      361 gtggtgctat tggagtattt ggagaggcag ctggatgagc cggcgccgca ggtttcaggt
      421 gttgaccagg ggcaagaact gctctgttgc aagatggcac tattgacacc agccccaggg
      481 tcacaaagta gccaatttca gctaatgaag gctctgctca agcatgaatc tgtgggatcc
      541 cagcctttac aagatagagt tctccaggtc cccgtgcttg cccatggagg atgctgcaga
      601 gaagataaag tggtagcttc taggcttact ccagagtccc aggggttgtt gaaagtggaa
      661 gatgtggccc tgaccctcac ccctgaatgg acacagcagg attcatctca ggggaatctc
      721 tgtagagatg aaaagcagga gaaccatggc agcctggtct ccctgggtga tgaaaaacag
      781 actaagagca gggacttgcc tccagctgag gagcttccag aaaaggagca tgggaagata
      841 tcgtgccacc tgagagaaga cattgcccag attcctacat gtgcagaagc tggtgaacag
      901 gagggcaggc tacaaagaaa gcagaaaaat gccacaggag ggaggcggca catctgccat
      961 gaatgtggaa agagttttgc tcaaagctca ggcctgagta aacacaggag aatccacact
     1021 ggtgagaaac cctacgaatg tgaagagtgt ggcaaagcct tcattgggag ctctgccctt
     1081 gtcattcatc agagagtcca cactggtgag aagccatatg agtgtgaaga atgtggtaag
     1141 gccttcagtc atagctcaga ccttatcaag catcagagaa cccacactgg ggagaagccc
     1201 tatgagtgtg atgactgtgg gaagaccttc agccagagct gcagcctcct tgaacatcac
     1261 agaatccaca ctggggagaa gccgtatcag tgcagtatgt gtggcaaagc ctttaggcga
     1321 agttcacatc tcctgagaca tcagaggatc catactgggg ataaaaatgt tcaggaacct
     1381 gagcagggag aggcctggaa aagtaggatg gaaagccagt tggaaaatgt tgaaactccc
     1441 atgtcttata aatgtaatga gtgtgaaaga agtttcactc agaatacagg cctcattgaa
     1501 catcaaaaaa tccacactgg tgagaaaccc tatcagtgta atgcgtgtgg aaaaggcttc
     1561 acccgaattt cataccttgt tcaacatcag agaagccatg tagggaaaaa catcctatca
     1621 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1681 tgtggaaagg acgatcgttc tactagggtg acctggtt
//
