LOCUS       sequence-282966-        4550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2769
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaccggg gtgtgacgag ttcctgccgc caccggagtg cccggttttt
       61 gagcctagct gggctgaatt ccaagacccg cttggctaca ttgcgaaaat aaggcccata
      121 gcagagaagt ctggcatctg caaaatccgc ccacccgcgg attggcagcc tccttttgca
      181 gtagaagttg acaatttcag atttactcct cgcgtccaaa ggctaaatga actggaggcc
      241 caaactagag tgaaattgaa ctatttggat cagattgcaa aattctggga aattcaaggc
      301 tcctctttaa agattcccaa tgtggagcgg aagatcttgg acctctacag ccttagtaag
      361 caatgtaaca cacacccgtt tgacaatgag gtaaaagata aggaatacaa gccccacagc
      421 atccccctta gacagtctgt gcagccttca aagttcagca gctacagtcg acgggcaaaa
      481 aggctacagc ctgatccaga gcctacagag gaggacattg agaagcatcc agagctaaag
      541 aagttacaga tatatgggcc aggtcccaaa atgatgggct tgggccttat ggctaaggat
      601 aaggataaga ctgtgcataa gaaagtcaca tgccccccaa ctgttacggt gaaggatgag
      661 caaagtggag gtgggaacgt gtcatcaaca ttgctcaagc agcacttgag cctagagccc
      721 tgcactaaga caaccatgca acttcgaaag aatcacagca gtgcccagtt tattgactca
      781 tatatttgcc aagtatgctc ccgtggggat gaagatgata agcttctttt ctgtgatggc
      841 tgtgatgaca attaccacat cttctgcttg ttaccacccc ttcctgaaat ccccagaggc
      901 atctggaggt gcccaaaatg tatcttggcg gagtgtaaac agcctcctga agcttttgga
      961 tttgaacagg ctacccagga gtacagtttg cagagttttg gtgaaatggc tgattccttc
     1021 aagtccgact acttcaacat gcctgtacat atggtgccta cagaacttgt agagaaggaa
     1081 ttctggaggc tggtgagcag cattgaggaa gacgtgacag ttgaatatgg agctgatatt
     1141 cattccaaag aatttggcag tggctttcct gtcagcaata gcaaacaaaa cttatctcct
     1201 gaggagaagg agtatgcgac cagtggttgg aacctgaatg tgatgccagt gctagatcag
     1261 tctgttctct gtcacatcaa tgcagacatc tcaggcatga aggtgccctg gctgtacgtg
     1321 ggcatggttt tctcagcatt ttgttggcat attgaggatc actggagtta ctctattaac
     1381 tatctgcatt ggggtgagcc gaagacctgg tatggtgtac cctccctggc agcagagcat
     1441 ttggaggagg tgatgaagat gctgacacct gagctgtttg atagccagcc tgatctccta
     1501 caccagcttg tcactctcat gaatcccaac actttgatgt cccatggtgt gccagttgtc
     1561 cgcacaaacc agtgtgcagg ggagtttgtc atcacttttc ctcgtgctta ccacagtggt
     1621 tttaaccaag gctacaattt tgctgaagct gtcaactttt gtactgctga ctggctacct
     1681 gctggacgcc agtgcattga acactaccgc cggctccggc gctattgtgt cttctcccac
     1741 gaggagctca tctgcaagat ggctgccttc ccagagacgt tggatctcaa tctagcagta
     1801 gctgtgcaca aggagatgtt cattatggtt caggaggagc gacgtctacg aaaggccctt
     1861 ttggagaagg gcgtcacgga ggctgagcga gaggcttttg agctgctccc agatgatgaa
     1921 cgccagtgca tcaagtgcaa gaccacgtgc ttcttgtcag ccctggcctg ctacgactgc
     1981 ccagatggcc ttgtatgcct ttcccacatc aatgacctct gcaagtgctc tagtagccga
     2041 cagtacctcc ggtatcggta caccttggat gagctcccca ccatgctgca taaactgaag
     2101 attcgggctg agtcttttga cacctgggcc aacaaagtgc gagtggcctt ggaggtggag
     2161 gatggccgta aacgcagctt tgaagagcta agggcactgg agtctgaggc tcgtgagagg
     2221 aggtttccta atagtgagct gcttcagcga ctgaagaact gcctgagtga ggtggaggct
     2281 tgtattgctc aagtcctggg gctggtcagt ggtcaggtgg ccaggatgga cactccacag
     2341 ctgaccttga ctgaactccg ggtccttctt gagcagatgg gcagcctgcc ctgtgccatg
     2401 catcagattg gggatgtcaa ggatgtcctg gaacaggtgg aggcctatca agctgaggct
     2461 cgtgaggctc tggccacact gccctctagt ccagggctat tgcggtccct gttggagagg
     2521 gggcagcagc tgggtgtaga ggtgcctgaa gcccatcagc ttcagcagca ggtggagcag
     2581 gcgcaatggc tagatgaagt gaagcaggcc ctggcccctt ctgctcacag gggctctctg
     2641 gtcatcatgc aggggctttt ggttatgggt gccaagatag cctccagccc ttctgtggac
     2701 aaggcccggg ctgagctgca agaactactg accattgcag agcgctggga agaaaaggct
     2761 catttctgcc tggaggccag gcagaagcat ccaccagcca cattggaagc cataattcgt
     2821 gagacagaaa acatccctgt tcacctgcct aacatccagg ctctcaaaga agctctgact
     2881 aaggcacaag cttggattgc tgatgtggat gagatccaaa atggtgacca ctacccctgt
     2941 ctagatgact tggagggcct ggtggctgtg ggccgggacc tgcctgtggg gctggaagag
     3001 ctgagacagc tagagctgca ggtattgaca gcacattcct ggagagagaa ggcctccaag
     3061 acctttctca agaagaattc ttgctacaca ctgcttgagg tgctttgccc gtgtgcagac
     3121 gctggctcag acagcaccaa gcgtagccgg tggatggaga aggcgctggg gttgtaccag
     3181 tgtgacacag agctgctggg gctgtctgca caggacctca gagacccagg ctctgtgatt
     3241 gtggccttca aggaagggga acagaaggag aaggagggta tcctgcagct gcgtcgcacc
     3301 aactcagcca agcccagtcc actggcacca tccctcatgg cctcttctcc gacttctatc
     3361 tgtgtgtgtg ggcaggtgcc agctggggtg ggagttctgc agtgtgacct gtgtcaggac
     3421 tggttccatg ggcagtgtgt gtcagtgccc catctcctca cctctccaaa gcccagtctc
     3481 acttcatctc cactgctagc ctggtgggaa tgggacacaa aattcctgtg tccactgtgt
     3541 atgcgctcac gacggccacg cctagagaca atcctagcct tgctggttgc cctgcagagg
     3601 ctgcccgtgc ggctgcctga gggtgaggcc cttcagtgtc tcacagagag ggccattggc
     3661 tggcaagacc gtgccagaaa ggctctggcc tctgaagatg tgactgctct gttgcgacag
     3721 ctggctgagc ttcgccaaca gctacaggcc aaacccagac cagaggaggc ctcagtctac
     3781 acttcagcca ctgcctgtga ccctatcaga gaaggcagtg gcaacaatat ttctaaggtc
     3841 caagggctgc tggagaatgg agacagtgtg accagtcctg agaacatggc tccaggaaag
     3901 ggctctgacc tggagctact gtcctcactg ttgccgcagt tgactggccc tgtgttggag
     3961 ctgcctgagg caatccgggc tcccctggag gagctcatga tggaagggga cctgcttgag
     4021 gtgaccctgg atgagaacca cagcatctgg cagctgctgc aggctggaca gcctccagac
     4081 ctggacagaa ttcgcacact tctggagctg gaaaaatttg aacatcaagg gagtcggaca
     4141 aggagccggg ctctggagag gcgacggcgg cggcagaagg tggatcaggg tagaaacgtt
     4201 gagaatcttg ttcaacagga gcttcagtca aaaagggctc ggagctcagg gattatgtct
     4261 caggtgggcc gagaagaaga acattatcag gagaaagcag accgtgaaaa tatgttcctg
     4321 acaccttcca cagaccacag ccctttcttg aaaggaaacc aaaatagctt acaacacaag
     4381 gattcaggct cttcagctgc ttgtccttct ttaatgcctt tgctacaact ctcctactct
     4441 gatgagcaac agttgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     4501 ctttatatat cttgtggaaa ggacgaatgt cgattgttca taacagatcg
//
