LOCUS       sequence-282969-        4721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2770
FEATURES             Location/Qualifiers
     source          1..4721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaccggg gtgtgacgag ttcctgccgc caccggagtg cccggttttt
       61 gagcctagct gggctgaatt ccaagacccg cttggctaca ttgcgaaaat aaggcccata
      121 gcagagaagt ctggcatctg caaaatccgc ccacccgcgg attggcagcc tccttttgca
      181 gtagaagttg acaatttcag atttactcct cgcgtccaaa ggctaaatga actggaggcc
      241 caaactagag tgaaattgaa ctatttggat cagattgcaa aattctggga aattcaaggc
      301 tcctctttaa agattcccaa tgtggagcgg aagatcttgg acctctacag ccttagtaag
      361 attgtgattg aggaaggtgg ctatgaagcc atctgcaagg atcgtcggtg ggctcgagtt
      421 gcccagcgtc tccactaccc accaggcaaa aacattggct ccctgctacg atcacattac
      481 gaacgcatta tttaccccta tgaaatgttt cagtctggag ccaaccatgt gcaatgtaac
      541 acacacccgt ttgacaatga ggtaaaagat aaggaataca agccccacag catccccctt
      601 agacagtctg tgcagccttc aaagttcagc agctacagtc gacgggcaaa aaggctacag
      661 cctgatccag agcctacaga ggaggacatt gagaagcatc cagagctaaa gaagttacag
      721 atatatgggc caggtcccaa aatgatgggc ttgggcctta tggctaagga taaggataag
      781 actgtgcata agaaagtcac atgcccccca actgttacgg tgaaggatga gcaaagtgga
      841 ggtgggaacg tgtcatcaac attgctcaag cagcacttga gcctagagcc ctgcactaag
      901 acaaccatgc aacttcgaaa gaatcacagc agtgcccagt ttattgactc atatatttgc
      961 caagtatgct cccgtgggga tgaagatgat aagcttcttt tctgtgatgg ctgtgatgac
     1021 aattaccaca tcttctgctt gttaccaccc cttcctgaaa tccccagagg catctggagg
     1081 tgcccaaaat gtatcttggc ggagtgtaaa cagcctcctg aagcttttgg atttgaacag
     1141 gctacccagg agtacagttt gcagagtttt ggtgaaatgg ctgattcctt caagtccgac
     1201 tacttcaaca tgcctgtaca tatggtgcct acagaacttg tagagaagga attctggagg
     1261 ctggtgagca gcattgagga agacgtgaca gttgaatatg gagctgatat tcattccaaa
     1321 gaatttggca gtggctttcc tgtcagcaat agcaaacaaa acttatctcc tgaggagaag
     1381 gagtatgcga ccagtggttg gaacctgaat gtgatgccag tgctagatca gtctgttctc
     1441 tgtcacatca atgcagacat ctcaggcatg aaggtgccct ggctgtacgt gggcatggtt
     1501 ttctcagcat tttgttggca tattgaggat cactggagtt actctattaa ctatctgcat
     1561 tggggtgagc cgaagacctg gtatggtgta ccctccctgg cagcagagca tttggaggag
     1621 gtgatgaaga tgctgacacc tgagctgttt gatagccagc ctgatctcct acaccagctt
     1681 gtcactctca tgaatcccaa cactttgatg tcccatggtg tgccagttgt ccgcacaaac
     1741 cagtgtgcag gggagtttgt catcactttt cctcgtgctt accacagtgg ttttaaccaa
     1801 ggctacaatt ttgctgaagc tgtcaacttt tgtactgctg actggctacc tgctggacgc
     1861 cagtgcattg aacactaccg ccggctccgg cgctattgtg tcttctccca cgaggagctc
     1921 atctgcaaga tggctgcctt cccagagacg ttggatctca atctagcagt agctgtgcac
     1981 aaggagatgt tcattatggt tcaggaggag cgacgtctac gaaaggccct tttggagaag
     2041 ggcgtcacgg aggctgagcg agaggctttt gagctgctcc cagatgatga acgccagtgc
     2101 atcaagtgca agaccacgtg cttcttgtca gccctggcct gctacgactg cccagatggc
     2161 cttgtatgcc tttcccacat caatgacctc tgcaagtgct ctagtagccg acagtacctc
     2221 cggtatcggt acaccttgga tgagctcccc accatgctgc ataaactgaa gattcgggct
     2281 gagtcttttg acacctgggc caacaaagtg cgagtggcct tggaggtgga ggatggccgt
     2341 aaacgcagct ttgaagagct aagggcactg gagtctgagg ctcgtgagag gaggtttcct
     2401 aatagtgagc tgcttcagcg actgaagaac tgcctgagtg aggtggaggc ttgtattgct
     2461 caagtcctgg ggctggtcag tggtcaggtg gccaggatgg acactccaca gctgaccttg
     2521 actgaactcc gggtccttct tgagcagatg ggcagcctgc cctgtgccat gcatcagatt
     2581 ggggatgtca aggatgtcct ggaacaggtg gaggcctatc aagctgaggc tcgtgaggct
     2641 ctggccacac tgccctctag tccagggcta ttgcggtccc tgttggagag ggggcagcag
     2701 ctgggtgtag aggtgcctga agcccatcag cttcagcagc aggtggagca ggcgcaatgg
     2761 ctagatgaag tgaagcaggc cctggcccct tctgctcaca ggggctctct ggtcatcatg
     2821 caggggcttt tggttatggg tgccaagata gcctccagcc cttctgtgga caaggcccgg
     2881 gctgagctgc aagaactact gaccattgca gagcgctggg aagaaaaggc tcatttctgc
     2941 ctggaggcca ggcagaagca tccaccagcc acattggaag ccataattcg tgagacagaa
     3001 aacatccctg ttcacctgcc taacatccag gctctcaaag aagctctgac taaggcacaa
     3061 gcttggattg ctgatgtgga tgagatccaa aatggtgacc actacccctg tctagatgac
     3121 ttggagggcc tggtggctgt gggccgggac ctgcctgtgg ggctggaaga gctgagacag
     3181 ctagagctgc aggtattgac agcacattcc tggagagaga aggcctccaa gacctttctc
     3241 aagaagaatt cttgctacac actgcttgag gtgctttgcc cgtgtgcaga cgctggctca
     3301 gacagcacca agcgtagccg gtggatggag aaggcgctgg ggttgtacca gtgtgacaca
     3361 gagctgctgg ggctgtctgc acaggacctc agagacccag gctctgtgat tgtggccttc
     3421 aaggaagggg aacagaagga gaaggagggt atcctgcagc tgcgtcgcac caactcagcc
     3481 aagcccagtc cactggcacc atccctcatg gcctcttctc cgacttctat ctgtgtgtgt
     3541 gggcaggtgc cagctggggt gggagttctg cagtgtgacc tgtgtcagga ctggttccat
     3601 gggcagtgtg tgtcagtgcc ccatctcctc acctctccaa agcccagtct cacttcatct
     3661 ccactgctag cctggtggga atgggacaca aaattcctgt gtccactgtg tatgcgctca
     3721 cgacggccac gcctagagac aatcctagcc ttgctggttg ccctgcagag gctgcccgtg
     3781 cggctgcctg agggtgaggc ccttcagtgt ctcacagaga gggccattgg ctggcaagac
     3841 cgtgccagaa aggctctggc ctctgaagat gtgactgctc tgttgcgaca gctggctgag
     3901 cttcgccaac agctacaggc caaacccaga ccagaggagg cctcagtcta cacttcagcc
     3961 actgcctgtg accctatcag agaaggcagt ggcaacaata tttctaaggt ccaagggctg
     4021 ctggagaatg gagacagtgt gaccagtcct gagaacatgg ctccaggaaa gggctctgac
     4081 ctggagctac tgtcctcact gttgccgcag ttgactggcc ctgtgttgga gctgcctgag
     4141 gcaatccggg ctcccctgga ggagctcatg atggaagggg acctgcttga ggtgaccctg
     4201 gatgagaacc acagcatctg gcagctgctg caggctggac agcctccaga cctggacaga
     4261 attcgcacac ttctggagct ggaaaaattt gaacatcaag ggagtcggac aaggagccgg
     4321 gctctggaga ggcgacggcg gcggcagaag gtggatcagg gtagaaacgt tgagaatctt
     4381 gttcaacagg agcttcagtc aaaaagggct cggagctcag ggattatgtc tcaggtgggc
     4441 cgagaagaag aacattatca ggagaaagca gaccgtgaaa atatgttcct gacaccttcc
     4501 acagaccaca gccctttctt gaaaggaaac caaaatagct tacaacacaa ggattcaggc
     4561 tcttcagctg cttgtccttc tttaatgcct ttgctacaac tctcctactc tgatgagcaa
     4621 cagttgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     4681 tcttgtggaa aggacgattt gagactcata ttggattaat t
//
