LOCUS       sequence-282975-        4736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2773
FEATURES             Location/Qualifiers
     source          1..4736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             340..351
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggaggcggc caccacactg cacccaggcc cgcgcccggc gctgcccctc
       61 gggggcccgg gcccgctggg cgagttcctg cctccacccg agtgcccggt cttcgaaccc
      121 agctgggaag agttcgcgga ccccttcgct ttcatccaca agatccggcc catagccgag
      181 cagactggca tctgtaaggt gcggccgccg ccggattggc agccaccatt tgcatgtgat
      241 gttgataaac ttcattttac gccacgtatc cagagactga atgaattgga ggcccaaact
      301 cgtgtaaaat tgaatttctt ggaccagatt gcaaagtact gggagttaca gggaagtact
      361 ctgaaaattc cacatgtgga gaggaagatc ttggacttat ttcagcttaa taagttagtt
      421 gcagaagaag gtggatttgc agttgtttgc aaggatagaa aatggaccaa aattgctacc
      481 aagatggggt ttgctcctgg caaagcagtg ggctcacata tcagagggca ttatgaacga
      541 attctcaacc cctacaactt attcctgtcc ggagacagcc taaggtgttt gcagaagcca
      601 aacctgacca cagacactaa ggacaaggag tacaaacccc atgatattcc ccagaggcag
      661 tctgtgcagc cttcggaaac gtgcccccca gcccgacgag caaaacgcat gagagcagag
      721 gccatgaata ttaaaataga acccgaggag acaacggaag ccagaactca taatctgaga
      781 cgtcgaatgg gttgtccaac tccaaaatgt gaaaatgaga aagaaatgaa gagtagcatc
      841 aagcaagaac ctattgagag gaaagattat attgtagaaa atgagaagga aaagcccaag
      901 agtcgatcta aaaaagccac caatgctgtg gacctgtatg tctgtctttt atgtggcagt
      961 ggcaatgatg aagaccggct actgttgtgt gatggctgtg atgacagtta ccataccttt
     1021 tgcttgatcc cacctctcca tgatgttccc aagggagact ggaggtgtcc taagtgtttg
     1081 gctcaggaat gtagtaagcc acaagaagca tttggctttg aacaagcagc cagggactat
     1141 accctccgta cttttgggga aatggcagat gcgttcaaat ctgattactt caacatgcca
     1201 gtccatatgg tccccacaga gcttgttgag aaagaatttt ggagactagt aagcactatt
     1261 gaggaggatg tcacagtgga atatggagct gacattgcct caaaggaatt tggcagtggc
     1321 tttcctgtcc gagatgggaa aatcaaactc tcacctgagg aagaggagta tcttgatagt
     1381 ggctggaatt tgaacaacat gccagtgatg gagcagtctg tccttgcaca tattactgct
     1441 gatatatgtg gcatgaaact tccttggttg tatgtgggaa tgtgcttttc ttcattctgt
     1501 tggcacattg aagaccactg gagctattca attaactact tgcactgggg tgagccaaaa
     1561 acctggtatg gagtcccagg gtatgctgct gagcagctag aaaatgtaat gaagaaacta
     1621 gctccagaac tctttgtgtc ccagccggat ctcctccatc agcttgtgac catcatgaac
     1681 cccaataccc tgatgactca tgaagtgcct gtttaccgaa ctaatcagtg tgctggggag
     1741 tttgtgatta catttccaag agcctaccac agtggtttta accagggttt taattttgct
     1801 gaggctgtta acttctgcac tgttgattgg ctgccattag gccgacagtg tgtggagcat
     1861 tatcgcttgc ttcatcgata ttgtgtgttt tcccacgatg agatgatctg caagatggct
     1921 tccaaggctg atgtattaga tgttgtagtg gcttcaactg ttcagaaaga catggccatt
     1981 atgattgagg atgagaaagc tttaagagaa actgtccgta aattgggagt gattgattcg
     2041 gaaagaatgg attttgagct gttgccagat gatgaacgtc agtgtgtaaa atgcaaaact
     2101 acatgcttca tgtctgccat ctcctgttct tgtaaacctg gccttcttgt ttgcctgcat
     2161 catgtaaaag aattgtgttc ctgtcctcct tacaaatata aattgcggta taggtacacg
     2221 ctggatgatc tctaccctat gatgaatgca ttgaagcttc gagcagaatc ttacaacgaa
     2281 tgggccttga atgtgaatga agctttggag gcaaagatca acaagaagaa aagccttgtc
     2341 agcttcaagg ctttaattga agaatctgaa atgaagaaat tcccagacaa tgatcttttg
     2401 cgacaccttc gcctagtcac acaggatgca gagaagtgtg cctctgttgc gcagcagttg
     2461 cttaatggca aaaggcaaac tagatatcga tctggtggag ggaaatccca aaatcagttg
     2521 acagtgaatg agctccggca gtttgtaaca cagctgtatg ctcttccatg tgtcctcagt
     2581 cagacaccat tactaaagga tctcttgaat cgtgtagaag attttcaaca gcatagtcag
     2641 aaactactct ctgaggaaac gcctagtgct gcggagctgc aggacttgct agatgtcagc
     2701 tttgaatttg atgttgaact tccacagctt gctgagatgc gtatccgttt ggaacaagcc
     2761 cgttggctag aagaggtgca gcaagcttgc ctagacccca gctcccttac tttagatgat
     2821 atgagacgtc tcatagacct aggggtaggg ctggccccgt attcagcagt ggagaaagct
     2881 atggcccggc tgcaggaact gctcacagtg tcagagcact gggacgacaa agccaagagt
     2941 ctcctcaagg ccaggccacg acattcattg aatagccttg ctacggcagt aaaggaaatc
     3001 gaagagatcc ctgcatatct gcccaatggt gcggctctga aagactcagt gcagagagcc
     3061 agagactggc ttcaggatgt agagggcctg caggctggag gacgtgtgcc agtgttagac
     3121 acactcatag aacttgttac acgaggccga tctatccccg tacatctgaa ttctttgcca
     3181 agactggaaa ccctagtagc tgaggttcag gcttggaaag aatgtgctgt taatacattc
     3241 ttgactgaga attctccata ttctctctta gaggtgctgt gtcctcgatg tgatattggc
     3301 cttttgggat tgaaaaggaa gcagagaaag ttaaaggagc ccttgccaaa tggaaagaaa
     3361 aaaagcacca aattagagag tctgagtgac ctggagagag ctttaactga aagcaaggag
     3421 actgcttcag ctatggcaac tcttggggaa gctcgcctaa gggaaatgga agccttgcag
     3481 tctctcagac tcgccaatga agggaaattg ctgtcgcctc tccaagatgt ggatataaaa
     3541 atctgcctat gtcagaaggc cccagctgcc cctatgattc aatgtgaact ctgcagggat
     3601 gctttccaca ccagttgtgt ggcggtaccc agtatttcac agggcctgcg aatctggctt
     3661 tgtccccatt gtcggaggtc agagaaacct ccattagaga aaattctgcc cctgctcgcc
     3721 tcccttcagc gtatccgagt tcgccttcct gagggagatg cacttcgata tatgattgaa
     3781 agaaccgtga actggcagca cagagcccag caactgcttt cgtcagggaa tcttaaattt
     3841 gtgcaagatc gagtgggctc aggactgtta tatagcagat ggcaagcctc agcaggacag
     3901 gtgtcagaca caaacaaggt atctcaacct cctggcacaa catcattttc tttgcctgat
     3961 gactgggaca acagaacctc atatttgcac tcccccttct caactggacg aagttgtatc
     4021 cccctccatg gtgttagtcc agaagtgaat gaactattga tggaagccca gctgctccag
     4081 gtatcccttc ctgaaattca ggaactttac cagactttac ttgcaaagcc aagccctgct
     4141 cagcagactg accgaagctc accagtgaga cccagcagtg agaagaatga ctgttgccga
     4201 gggaagcgag atggaattaa cagtcttgag agaaaactga agagacgcct ggaaagagag
     4261 ggcctctcca gtgagcggtg ggaacgagtt aagaaaatgc ggacccccaa aaagaagaaa
     4321 atcaaactga gccaccccaa ggacatgaac aatttcaagt tagagagaga gcgtagctat
     4381 gaattagttc gttctgctga aactcattcc ctgccctcag acacatccta ttccgaacag
     4441 gaagactctg aggatgaaga tgccatctgc ccagctgtga gctgcctgca gccagaagga
     4501 gatgaggtgg actgggtcca gtgtgatggc agctgcaatc agtggtttca tcaggtctgt
     4561 gttggtgtct ccccagagat ggcagagaaa gaagactaca tctgtgtgcg ctgtactgtg
     4621 aaggacgcac caagccgaaa gtagtaatga actagtccgt aacttgaaag tatttcgatt
     4681 tcttggcttt atatatcttg tggaaaggac gattgggtac cgggttctat gctccg
//
