LOCUS       sequence-282984-        1964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2785
FEATURES             Location/Qualifiers
     source          1..1964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaggc ccgcacatcc ctgtctgccc actgtcgggg cccgctggcc
       61 actggcctgc acccagacct ggacctcccg ggccgaagcc tcgccacccc tgcgccttcc
      121 tgctaccttc tgggcagcga acccagctct ggcctgggcc tccagcccga gacccacctc
      181 cccgagggca gcctgaagcg gtgctgcgtc ttgggcctac cccccacctc cccagcctcc
      241 tcctcaccct gtgcctcctc cgacgtcacc tccatcatcc gctcctccca gacgtctctg
      301 gtcacctgtg taaatggact ccggagcccc cctctgacgg gagatctggg gggcccttcc
      361 aagcgggccc ggcctggccc tgcatcgacg gacagccatg agggcagctt gcaacttgaa
      421 gcctgccgga aggcgagctt cctgaagcag gaacccgcgg atgagttttc agagctcttt
      481 gggcctcacc agcagggcct gccgcccccc tatcccctgt ctcagttgcc gcctggccca
      541 agccttggag gcctggggct gggcctggca ggcagggtgg tggccgggcg gcaggcgtgc
      601 cgctgggtgg actgctgtgc agcctatgag cagcaggagg agctggtgcg gcacatcgag
      661 aagagccaca tcgaccagcg caagggcgag gacttcacct gcttctgggc tggctgcgtg
      721 cgccgctaca agcccttcaa cgcccgctac aagctgctca tccacatgcg agtgcactcg
      781 ggcgagaagc ccaacaagtg catgtttgaa ggctgcagca aggccttctc acggctggag
      841 aacctcaaga tccacctgag gagccacacg ggcgagaagc cgtacctgtg ccagcacccg
      901 ggttgccaga aggccttcag caactccagc gaccgcgcca agcaccagcg cacccaccta
      961 gacacgaagc cgtacgcctg tcagatccct ggctgctcca agcgctacac agaccccagc
     1021 tccctccgca agcacgtcaa ggcccattca gccaaagagc agcaggtgcg taagaagctg
     1081 catgcgggcc ctgacaccga ggccgacgtc ctgaccgagt gtctggtcct gcagcagctc
     1141 cacacgtcca cacagctggc tgccagcgac ggcaagggtg gctgtggcct gggccaggag
     1201 ctgctcccag gtgtgtatcc tggctccatc accccccata acggacttgc atcgggcctc
     1261 ctgcccccag cgcacgacgt accttccagg caccacccgc tggatgccac caccagttcc
     1321 caccaccatc tgtcccctct gcccatggct gagagcaccc gggatgggtt ggggcccggc
     1381 ctcctctcac caatagtcag ccccctgaag gggctggggc caccgccgct gcccccatcc
     1441 tctcagagcc attctccggg gggccagccc ttccccacac tccccagcaa gccgtcctac
     1501 ccacccttcc agagccctcc acccccgcct ctgcccagcc cacaaggtta ccagggcagt
     1561 ttccactcca tccagagttg cttcccctat ggcgactgct accggatggc tgaaccagca
     1621 gccggtgggg acggactggt cggggagacc cacggtttca accccctgcg gcccaatggc
     1681 taccacagcc tcagcacgcc cttgcctgcc acaggctatg aggccctggc tgaggcctca
     1741 tgccccacag cgctgccaca gcagccatct gaagatgtgg tgtccagcgg ccccgaggac
     1801 tgtggcttct tccccaatgg agcctttgac cactgcctgg gccacatccc ctccatctac
     1861 acagacacct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1921 atatcttgtg gaaaggacga aagactcctc acggagtcga ctta
//
