LOCUS       sequence-28299-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Meylan E, Dooley AL, Feldser DM, Shen L, Turk E, Ouyang C, Jacks T
  TITLE     Requirement for NF-kappaB signalling in a mouse model of lung 
            adenocarcinoma.
  JOURNAL   Nature. 2009 Nov 5;462(7269):104-7. doi: 10.1038/nature08462. Epub 
            2009 Oct 21.
  PUBMED    19847165
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08462"; journalName: "Nature"; date: "2009-11-5- 5"; 
            volume: "462"; issue: "7269"; pages: "104-7"
COMMENT     Sequence Label: pSicoR (Puro) cRel shRNA2
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        152..651
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(187..210)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     584..603
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(695..714)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gtgtgttttg agactataaa tatcccttgg agaaagcctt gtttgcctca tcctcatgat
       61 ttattcaaga gataaatcat gaggatgagg cttttttctc gagtactagg atccattagg
      121 cggccgaatt cgtcgacctc gaaattctac cgggtagggg aggcgctttt cccaaggcag
      181 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
      241 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
      301 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
      361 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
      421 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
      481 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
      541 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
      601 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcatc
      661 catctagatc tcgatcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg
      721 cctcgccacc cgcgacgacg tccccagggn cgtacgcacc ctcgccgccg cgttcgccga
      781 c
//
