LOCUS       sequence-282990-        2135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2799
FEATURES             Location/Qualifiers
     source          1..2135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagga gaagaagctg aagcttagca acactgtgct gccctcggag
       61 tccatgaagg tggtggctga atccatgggc atcgcccaga ttcaggagga gacctgccag
      121 ctgctaacgg atgaggtcag ctaccgcatc aaagagatcg cacaggatgc cttgaagttc
      181 atgcacatgg ggaagcggca gaagctcacc accagtgaca ttgactacgc cttgaagcta
      241 aagaatgtcg agccactcta tggcttccac gcccaggagt tcattccttt ccgcttcgcc
      301 tctggtgggg gccgggagct ttacttctat gaggagaagg aggttgatct gagcgacatc
      361 atcaataccc ctctgccccg ggtgcccctg gacgtctgcc tcaaagctca ttggctgagc
      421 atcgagggct gccagccagc tatccccgag aacccgcccc cagctcccaa agagcaacag
      481 aaggctgaag ccacagaacc cctgaagtca gccaagccag gccaggagga agacggaccc
      541 ctgaagggca aaggtcaagg ggccaccaca gccgacggca aagggaaaga gaagaaggcg
      601 ccgcccttgc tggagggggc ccccttgcga ctgaagcccc ggagcatcca cgagttgtct
      661 gtggagcagc agctctacta caaggagatc accgaggcct gcgtgggctc ctgcgaggcc
      721 aagagggcgg aagccctgca aagcattgcc acggaccctg gactgtatca gatgctgcca
      781 cggttcagta cctttatctc ggagggggtc cgtgtgaacg tggttcagaa caacctggcc
      841 ctactcatct acctgatgcg tatggtgaaa gcgctgatgg acaaccccac gctctatcta
      901 gaaaaatacg tccatgagct gattccagct gtgatgacct gcatcgtgag cagacagttg
      961 tgcctgcgac cagatgtgga caatcactgg gcactccgag actttgctgc ccgcctggtg
     1021 gcccagatct gcaagcattt tagcacaacc actaacaaca tccagtcccg gatcaccaag
     1081 accttcacca agagctgggt ggacgagaag acgccctgga cgactcgtta tggctccatc
     1141 gcaggcttgg ctgagctggg acacgatgtt atcaagactc tgattctgcc ccggctgcag
     1201 caggaagggg agcggatccg cagtgtgctg gacggccctg tgctgagcaa cattgaccgg
     1261 attggagcag accatgtgca gagcctcctg ctgaaacact gtgctcctgt tctggcaaag
     1321 ctgcgcccac cgcctgacaa tcaggacgcc tatcgggcag aattcgggtc ccttgggccc
     1381 ctcctctgct cccaggtggt caaggctcgg gcccaggctg ctctgcaggc tcagcaggtc
     1441 aacaggacca ctctgaccat cacgcagccc cggcccacgc tgaccctctc gcaggcccca
     1501 cagcctggcc ctcgcacccc tggcttgctg aaggttcctg gctccatcgc acttcctgtc
     1561 cagacactgg tgtctgcacg agcggctgcc ccaccacagc cttcccctcc tccaaccaag
     1621 tttattgtaa tgtcatcgtc ctccagcgcc ccatccaccc agcaggtcct gtccctcagc
     1681 acctcggccc ccggctcagg ttccaccacc acttcgcccg tcaccaccac cgtccccagc
     1741 gtgcagccca tcgtcaagtt ggtctccacc gccaccaccg caccccccag cactgctccc
     1801 tctggtcctg ggagtgtcca gaagtacatc gtggtctcac ttcccccaac aggggagggc
     1861 aaaggaggcc ccacctccca tccttctcca gttcctcccc cggcatcgtc cccgtcccca
     1921 ctcagcggca gtgccctttg tggggggaag caggaggctg gggacagtcc ccctccagct
     1981 ccagggactc caaaagccaa tggctcccag cccaactccg gctcccctca gcctgctccg
     2041 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2101 ggaaaggacg agactgcgca gcggatctgc actaa
//
