LOCUS       sequence-282993-        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2801
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacctgtg gaactgggat gaggcatccc cacaggaagt gcctccaggg
       61 aacaagctgg cagggcttgg taggctgccg aggctgccac aacgtgtgtg gggagggtgt
      121 ccaggtgggg cctctgctga ccctaacccc ttatcgcctg cagaaggagc caaattaggc
      181 ttctgtttcc ctgatctggc actccaaggg gacacgccga cagcgacagc agagacatgc
      241 tggaaaggta caagctcatc cctggcaagc ttcccacagc tggactgggg ctccgcgtta
      301 ctgcacccag aagttccatg gggggcggag cccgactctc aggctcttcc gtggtccggg
      361 gactggacag acatggcgtg cacagcctgg gactcttgga gcggcgcctc gcagaccctg
      421 ggccccgccc ctctcggccc gggccccatc cccgccgccg gctccgaagg cgccgcgggc
      481 cagaactgcg tccccgtggc gggagaggcc acctcgtggt cgcgcgccca ggccgccggg
      541 agcaacacca gctgggactg ttctgtgggg cccgacggcg atacctactg gggcagtggc
      601 ctgggcgggg agccgcgcac ggactgtacc atttcgtggg gcgggcccgc gggcccggac
      661 tgtaccacct cctggaaccc ggggctgcat gcgggtggca ccacctcttt gaagcggtac
      721 cagagctcag ctctcaccgt ttgctccgaa ccgagcccgc agtcggaccg tgccagtttg
      781 gctcgatgcc ccaaaactaa ccaccgaggt cccattcagc tgtggcagtt cctcctggag
      841 ctgctccacg acggggcgcg tagcagctgc atccgttgga ctggcaacag ccgcgagttc
      901 cagctgtgcg accccaaaga ggtggctcgg ctgtggggcg agcgcaagag aaagccgggc
      961 atgaattacg agaagctgag ccggggcctt cgctactact atcgccgcga catcgtgcgc
     1021 aagagcgggg ggcgaaagta cacgtaccgc ttcgggggcc gcgtgcccag cctagcctat
     1081 ccggactgtg cgggaggcgg acggggagca gagacacaat agtaatgaac tagtccgtaa
     1141 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tgcttccgtt
     1201 gctttctgtg cact
//
