LOCUS       sequence-283017-        1661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2823
FEATURES             Location/Qualifiers
     source          1..1661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgctga tgagggtcaa gacatgtccc aagtttcagg gaaggaaagc
       61 ccccctgtaa gcgatactcc agatgagggc gatgagccca tgccgatccc cgaggacctc
      121 tccaccacct cgggaggaca gcaaagctcc aagagtgaca gagtcgtggc cagtaatgtt
      181 aaagtagaga ctcagagtga tgaagagaat gggcgtgcct gtgaaatgaa tggggaagaa
      241 tgtgcggagg atttacgaat gcttgatgcc tcgggagaga aaatgaatgg ctcccacagg
      301 gaccaaggca gctcggcttt gtcgggagtt ggaggcattc gacttcctaa cggaaaacta
      361 aagtgtgata tctgtgggat catttgcatc gggcccaatg tgctcatggt tcacaaaaga
      421 agccacactg gagaacggcc cttccagtgc aatcagtgcg gggcctcatt cacccagaag
      481 ggcaacctgc tccggcacat caagctgcat tccggggaga agcccttcaa atgccacctc
      541 tgcaactacg cctgccgccg gagggacgcc ctcactggcc acctgaggac gcactccgtt
      601 ggtaaacctc acaaatgtgg atattgtggc cgaagctata aacagcgaag ctctttagag
      661 gaacataaag agcgctgcca caactacttg gaaagcatgg gccttccggg cacactgtac
      721 ccagtcatta aagaagaaac taatcacagt gaaatggcag aagacctgtg caagatagga
      781 tcagagagat ctctcgtgct ggacagacta gcaagtaacg tcgccaaacg taagagctct
      841 atgcctcaga aatttcttgg ggacaagggc ctgtccgaca cgccctacga cagcagcgcc
      901 agctacgaga aggagaacga aatgatgaag tcccacgtga tggaccaagc catcaacaac
      961 gccatcaact acctgggggc cgagtccctg cgcccgctgg tgcagacgcc cccgggcggt
     1021 tccgaggtgg tcccggtcat cagcccgatg taccagctgc acaagccgct cgcggagggc
     1081 accccgcgct ccaaccactc ggcccaggac agcgccgtgg agaacctgct gctgctctcc
     1141 aaggccaagt tggtgccctc ggagcgcgag gcgtccccga gcaacagctg ccaagactcc
     1201 acggacaccg agagcaacaa cgaggagcag cgcagcggtc tcatctacct gaccaaccac
     1261 atcgccccgc acgcgcgcaa cgggctgtcg ctcaaggagg agcaccgcgc ctacgacctg
     1321 ctgcgcgccg cctccgagaa ctcgcaggac gcgctccgcg tggtcagcac cagcggggag
     1381 cagatgaagg tgtacaagtg cgaacactgc cgggtgctct tcctggatca cgtcatgtac
     1441 accatccaca tgggctgcca cggcttccgt gatccttttg agtgcaacat gtgcggctac
     1501 cacagccagg accggtacga gttctcgtcg cacataacgc gaggggagca ccgcttccac
     1561 atgagctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1621 tcttgtggaa aggacgaact gcactcggag ttagtgatca t
//
