LOCUS       sequence-283020-        1400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2824
FEATURES             Location/Qualifiers
     source          1..1400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgctga tgagggtcaa gacatgtccc aagtttcagg gaaggaaagc
       61 ccccctgtaa gcgatactcc agatgagggc gatgagccca tgccgatccc cgaggacctc
      121 tccaccacct cgggaggaca gcaaagctcc aagagtgaca gagtcgtggg agaacggccc
      181 ttccagtgca atcagtgcgg ggcctcattc acccagaagg gcaacctgct ccggcacatc
      241 aagctgcatt ccggggagaa gcccttcaaa tgccacctct gcaactacgc ctgccgccgg
      301 agggacgccc tcactggcca cctgaggacg cactccgttg gtaaacctca caaatgtgga
      361 tattgtggcc gaagctataa acagcgaagc tctttagagg aacataaaga gcgctgccac
      421 aactacttgg aaagcatggg ccttccgggc acactgtacc cagtcattaa agaagaaact
      481 aatcacagtg aaatggcaga agacctgtgc aagataggat cagagagatc tctcgtgctg
      541 gacagactag caagtaacgt cgccaaacgt aagagctcta tgcctcagaa atttcttggg
      601 gacaagggcc tgtccgacac gccctacgac agcagcgcca gctacgagaa ggagaacgaa
      661 atgatgaagt cccacgtgat ggaccaagcc atcaacaacg ccatcaacta cctgggggcc
      721 gagtccctgc gcccgctggt gcagacgccc ccgggcggtt ccgaggtggt cccggtcatc
      781 agcccgatgt accagctgca caagccgctc gcggagggca ccccgcgctc caaccactcg
      841 gcccaggaca gcgccgtgga gaacctgctg ctgctctcca aggccaagtt ggtgccctcg
      901 gagcgcgagg cgtccccgag caacagctgc caagactcca cggacaccga gagcaacaac
      961 gaggagcagc gcagcggtct catctacctg accaaccaca tcgccccgca cgcgcgcaac
     1021 gggctgtcgc tcaaggagga gcaccgcgcc tacgacctgc tgcgcgccgc ctccgagaac
     1081 tcgcaggacg cgctccgcgt ggtcagcacc agcggggagc agatgaaggt gtacaagtgc
     1141 gaacactgcc gggtgctctt cctggatcac gtcatgtaca ccatccacat gggctgccac
     1201 ggcttccgtg atccttttga gtgcaacatg tgcggctacc acagccagga ccggtacgag
     1261 ttctcgtcgc acataacgcg aggggagcac cgcttccaca tgagctagta atgaactagt
     1321 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacca
     1381 tactcacgaa actccagatg
//
