LOCUS       sequence-283023-        1232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2826
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgctga tgagggtcaa gacatgtccc aagtttcagg gaaggaaagc
       61 ccccctgtaa gcgatactcc agatgagggc gatgagccca tgccgatccc cgaggacctc
      121 tccaccacct cgggaggaca gcaaagctcc aagagtgaca gagtcgtggc cagtaatgtt
      181 aaagtagaga ctcagagtga tgaagagaat gggcgtgcct gtgaaatgaa tggggaagaa
      241 tgtgcggagg atttacgaat gcttgatgcc tcgggagaga aaatgaatgg ctcccacagg
      301 gaccaaggca gctcggcttt gtcgggagtt ggaggcattc gacttcctaa cggaaaacta
      361 aagtgtgata tctgtgggat catttgcatc gggcccaatg tgctcatggt tcacaaaaga
      421 agccacactg gggacaaggg cctgtccgac acgccctacg acagcagcgc cagctacgag
      481 aaggagaacg aaatgatgaa gtcccacgtg atggaccaag ccatcaacaa cgccatcaac
      541 tacctggggg ccgagtccct gcgcccgctg gtgcagacgc ccccgggcgg ttccgaggtg
      601 gtcccggtca tcagcccgat gtaccagctg cacaagccgc tcgcggaggg caccccgcgc
      661 tccaaccact cggcccagga cagcgccgtg gagaacctgc tgctgctctc caaggccaag
      721 ttggtgccct cggagcgcga ggcgtccccg agcaacagct gccaagactc cacggacacc
      781 gagagcaaca acgaggagca gcgcagcggt ctcatctacc tgaccaacca catcgccccg
      841 cacgcgcgca acgggctgtc gctcaaggag gagcaccgcg cctacgacct gctgcgcgcc
      901 gcctccgaga actcgcagga cgcgctccgc gtggtcagca ccagcgggga gcagatgaag
      961 gtgtacaagt gcgaacactg ccgggtgctc ttcctggatc acgtcatgta caccatccac
     1021 atgggctgcc acggcttccg tgatcctttt gagtgcaaca tgtgcggcta ccacagccag
     1081 gaccggtacg agttctcgtc gcacataacg cgaggggagc accgcttcca catgagctag
     1141 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1201 aaggacgacg gagtatcctt ccatgacact ct
//
