LOCUS       sequence-283038-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2844
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaactga acagccagaa gaaaccttcc ctaacactga aaccaatggt
       61 gaatttggta aacgccctgc agaagatatg gaagaggaac aagcatttaa aagatctaga
      121 aacactgatg agatggttga attacgcatt ctgcttcaga gcaagaatgc tggggcagtg
      181 attggaaaag gaggcaagaa tattaaggct ctccgtacag actacaatgc cagtgtttca
      241 gtcccagaca gcagtggccc cgagcgcata ttgagtatca gtgctgatat tgaaacaatt
      301 ggagaaattc tgaagaaaat catccctacc ttggaagagg gcctgcagtt gccatcaccc
      361 actgcaacca gccagctccc gctcgaatct gatgctgtgg aatgcttaaa ttaccaacac
      421 tataaaggaa gtgactttga ctgcgagttg aggctgttga ttcatcagag tctagcagga
      481 ggaattattg gggtcaaagg tgctaaaatc aaagaacttc gagagaacac tcaaaccacc
      541 atcaagcttt tccaggaatg ctgtcctcat tccactgaca gagttgttct tattggagga
      601 aaacccgata gggttgtaga gtgcataaag atcatccttg atcttatatc tgagtctccc
      661 atcaaaggac gtgcacagcc ttatgatccc aatttttacg atgaaaccta tgattatggt
      721 ggttttacaa tgatgtttga tgaccgtcgc ggacgcccag tgggatttcc catgcgggga
      781 agaggtggtt ttgacagaat gcctcctggt cggggtgggc gtcccatgcc tccatctaga
      841 agagattatg atgatatgag ccctcgtcga ggaccacctc cccctcctcc cggacgaggc
      901 ggccggggtg gtagcagagc tcggaatctt cctcttcctc caccaccacc acctagaggg
      961 ggagacctca tggcctatga cagaagaggg agacctggag accgttacga cggcatggtt
     1021 ggtttcagtg ctgatgaaac ttgggactct gcaatagata catggagccc atcagaatgg
     1081 cagatggctt atgaaccaca gggtggctcc ggatatgatt attcctatgc agggggtcgt
     1141 ggctcatatg gtgatcttgg tggacctatt attactacac aagtaactat tcccaaagat
     1201 ttggctggat ctattattgg caaaggtggt cagcggatta aacaaatccg tcatgagtcg
     1261 ggagcttcga tcaaaattga tgagccttta gaaggatccg aagatcggat cattaccatt
     1321 acaggaacac aggaccagat acagaatgca cagtatttgc tgcagaacag tgtgaagcag
     1381 tattctggaa agtttttcta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgaa acagactaga ggccgacatt ggg
//
