LOCUS       sequence-283062-        3527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2861
FEATURES             Location/Qualifiers
     source          1..3527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgacag cggcgagctg ggtggtctgg agaccatgga gaccctcacg
       61 gagctgggcg acgagctgac cctgggagac atcgacgaga tgctgcaatt tgtcagtaat
      121 caagtgggag agttccctga cttgttttca gaacagctgt gtagctcctt tcctggcagt
      181 ggtggtagtg gtagcagcag cggcagcagt ggcagcagca gcagcagcag caatggcagg
      241 ggcagcagca gcggagctgt ggacccttca gtgcaacggt cattcaccca ggtcacatta
      301 ccttccttct ctccctcggc ggcctcccca caggctccaa ctctgcaagt caaggtttct
      361 cccacctcag ttcccaccac acccagggca actcctattc ttcagccccg cccccagccc
      421 cagcctcaac ctcaaactca gctgcaacaa cagacggtaa tgatcacgcc aacattcagc
      481 accactccgc agacgaggat catccagcag cctttgatat accagaatgc agctactagc
      541 tttcaagtcc ttcagcctca agtccaaagc ctggtgacat cctcccaggt acagccggtc
      601 accattcagc agcaggtgca gacagtacag gcccagcggg tgctgacaca aacggccaat
      661 ggcacgctgc agacccttgc cccggctacg gtgcagacag ttgctgcgcc acaggtgcag
      721 caggtcccgg tcctggtcca gcctcagatc atcaagacag attcccttgt tttgaccaca
      781 ctgaagacag atggcagccc tgttatggct gcggtccaga acccggccct caccgccctc
      841 accaccccta tccagacggc tgcccttcaa gtaccaaccc tggtgggcag cagtgggacc
      901 attctgacca caatgcctgt aatgatgggg caagagaaag tgcccattaa gcaggtacct
      961 gggggagtca agcagcttga gccccccaaa gaaggagaaa ggcggacaac ccataatatc
     1021 attgagaaac gatatcgctc ctccatcaat gacaaaatca tcgaattgaa agacctggtc
     1081 atggggacag acgccaagat gcacaagtct ggcgttctga ggaaggccat tgattacatc
     1141 aaatacttgc agcaggtcaa tcataaactg cgccaggaga acatggtgct gaagctggca
     1201 aatcaaaaga acaagcttct aaagggcatc gacctaggca gtctggtgga caatgaggtg
     1261 gacctgaaga tcgaggactt taatcagaat gtccttctga tgtccccccc agcctctgac
     1321 tcagggtccc aggctggctt ctctccctac tccattgact ctgagccagg aagccctcta
     1381 ttggatgatg caaaggtcaa agatgagcca gactctcctc ctgtggcgct gggcatggta
     1441 gaccgctcac ggattcttct gtgtgtcctc accttcctgt gcctctcctt taaccccctg
     1501 acttccctgc tgcagtgggg aggggcccac gactctgacc agcacccaca ctcaggctct
     1561 ggccgcagtg tcctgtcatt cgagtcaggt tctgggggct ggtttgactg gatgatgcct
     1621 actcttctct tatggctggt aaatggtgtg attgtcctga gcgtctttgt gaagctgctg
     1681 gttcatgggg agccagtgat ccggccacac tcgcgctcct cggtcacctt ctggaggcac
     1741 cggaaacagg cagatctgga tctcgccaga ggagattttg cagctgctgc cggcaaccta
     1801 caaacctgcc tggcagtttt gggccgggca ctgcccacct cccgcctgga cctggcctgc
     1861 agcctctcct ggaacgtgat ccgctacagc ctgcagaagc tacgcctggt gcgctggctg
     1921 ctcaagaaag tcttccagtg ccggcgggcc acgccagcca ctgaggcagg ctttgaagac
     1981 gaagctaaga ccagcgcccg ggatgcggct ctggcctatc accggctgca ccagctgcac
     2041 atcacaggga agcttcctgc aggatccgcc tgttccgatg tacacatggc gttgtgtgcc
     2101 gtgaacctgg ctgaatgtgc agaggagaag atcccaccga gcacactggt tgagatccat
     2161 ctgactgctg ccatggggct caagacccgg tgtggaggca agctgggctt cctggccagc
     2221 tacttcctca gccgagccca gagcctgtgt ggccccgagc acagtgctgt tcctgactcc
     2281 ctgcgctggc tctgccaccc cctgggccag aagtttttca tggagcggag ctggtctgtg
     2341 aagtcagctg ccaaggagag tctatactgt gcccagagga acccagctga ccccattgcg
     2401 caggtccacc aggccttctg caagaacctg ctggagcgag ctatagagtc cttggtgaaa
     2461 cctcaggcca agaagaaggc tggagaccag gaagaagaga gctgtgaatt ctccagtgct
     2521 ctggagtact tgaaattact tcattctttt gtggactctg tgggggttat gagcccccca
     2581 ctctccagga gctccgtgct caagtccgcc ctgggtccag acatcatctg tcggtggtgg
     2641 acgtctgcaa tcactgtggc catcagctgg ctccagggag acgatgcagc tgtgcgctct
     2701 cattttacca aagtggaacg catccccaag gccctggaag tgacagagag ccccctggtg
     2761 aaggccatct tccatgcctg cagagccatg catgcctcac tccctgggaa agcagatggg
     2821 cagcagagtt ccttctgcca ttgcgagagg gccagtggcc acctatggag cagcctcaac
     2881 gtcagtgggg ccacctctga ccctgccctc aaccacgtgg tccagctgct cacctgtgac
     2941 ctgctactgt cgctacggac agcgctctgg caaaaacagg ccagtgccag ccaggctgtg
     3001 ggggagacct accacgcgtc aggcgctgaa ctggcgggct tccaacggga cctgggcagc
     3061 ctgcgcaggc tggcacacag cttccgccca gcataccgca aggtgttcct gcatgaagcc
     3121 accgtgcgcc tgatggcagg agccagcccc acccgcaccc accagctgct ggaacacagc
     3181 ctgcggcggc gcaccacgca gagcaccaag cacggagagg tggatgcctg gcccggccag
     3241 cgagagcggg ccaccgccat cctgctggcc tgccgccacc tgcccctctc cttcctctcc
     3301 tccccgggcc agcgggcagt gctgctggcc gaagctgccc gcaccctgga gaaggtgggc
     3361 gaccggcgct cctgcaacga ctgccagcag atgattgtta agctgggtgg tggcactgcc
     3421 attgccgcct cctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3481 tatatatctt gtggaaagga cgatcggaac ttggctatcc tgcttct
//
