LOCUS       sequence-283065-        3635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2862
FEATURES             Location/Qualifiers
     source          1..3635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgagcc acccttcagc gaggcggctt tggagcaggc gctgggcgag
       61 ccgtgcgatc tggacgcggc gctgctgacc gacatcgaag gtgaagtcgg cgcggggagg
      121 ggtagggcca acggcctgga cgccccaagg gcgggcgcag atcgcggagc catggattgc
      181 actttcgaag acatgcttca gcttatcaac aaccaagaca gtgacttccc tggcctattt
      241 gacccaccct atgctgggag tggggcaggg ggcacagacc ctgccagccc cgataccagc
      301 tccccaggca gcttgtctcc acctcctgcc acattgagct cctctcttga agccttcctg
      361 agcgggccgc aggcagcgcc ctcacccctg tcccctcccc agcctgcacc cactccattg
      421 aagatgtacc cgtccatgcc cgctttctcc cctgggcctg gtatcaagga agagtcagtg
      481 ccactgagca tcctgcagac ccccacccca cagcccctgc caggggccct cctgccacag
      541 agcttcccag ccccagcccc accgcagttc agctccaccc ctgtgttagg ctaccccagc
      601 cctccgggag gcttctctac aggaagccct cccgggaaca cccagcagcc gctgcctggc
      661 ctgccactgg cttccccgcc aggggtcccg cccgtctcct tgcacaccca ggtccagagt
      721 gtggtccccc agcagctact gacagtcaca gctgccccca cggcagcccc tgtaacgacc
      781 actgtgacct cgcagatcca gcaggtcccg gtcctgctgc agccccactt catcaaggca
      841 gactcgctgc ttctgacagc catgaagaca gacggagcca ctgtgaaggc ggcaggtctc
      901 agtcccctgg tctctggcac cactgtgcag acagggcctt tgccgaccct ggtgagtggc
      961 ggaaccatct tggcaacagt cccactggtc gtagatgcgg agaagctgcc tatcaaccgg
     1021 ctcgcagctg gcagcaaggc cccggcctct gcccagagcc gtggagagaa gcgcacagcc
     1081 cacaacgcca ttgagaagcg ctaccgctcc tccatcaatg acaaaatcat tgagctcaag
     1141 gatctggtgg tgggcactga ggcaaagctg aataaatctg ctgtcttgcg caaggccatc
     1201 gactacattc gctttctgca acacagcaac cagaaactca agcaggagaa cctaagtctg
     1261 cgcactgctg tccacaaaag caaatctctg aaggatctgg tgtcggcctg tggcagtgga
     1321 gggaacacag acgtgctcat ggagggcgtg aagactgagg tggaggacac actgacccca
     1381 cccccctcgg atgctggctc acctttccag agcagcccct tgtcccttgg cagcaggggc
     1441 agtggcagcg gtggcagtgg cagtgactcg gagcctgaca gcccagtctt tgaggacagc
     1501 aaggcaaagc cagagcagcg gccgtctctg cacagccggg gcatgctgga ccgctcccgc
     1561 ctggccctgt gcacgctcgt cttcctctgc ctgtcctgca accccttggc ctccttgctg
     1621 ggggcccggg ggcttcccag cccctcagat accaccagcg tctaccatag ccctgggcgc
     1681 aacgtgctgg gcaccgagag cagagatggc cctggctggg cccagtggct gctgccccca
     1741 gtggtctggc tgctcaatgg gctgttggtg ctcgtctcct tggtgcttct ctttgtctac
     1801 ggtgagccag tcacacggcc ccactcaggc cccgccgtgt acttctggag gcatcgcaag
     1861 caggctgacc tggacctggc ccggggagac tttgcccagg ctgcccagca gctgtggctg
     1921 gccctgcggg cactgggccg gcccctgccc acctcccacc tggacctggc ttgtagcctc
     1981 ctctggaacc tcatccgtca cctgctgcag cgtctctggg tgggccgctg gctggcaggc
     2041 cgggcagggg gcctgcagca ggactgtgct ctgcgagtgg atgctagcgc cagcgcccga
     2101 gacgcagccc tggtctacca taagctgcac cagctgcaca ccatggggaa gcacacaggc
     2161 gggcacctca ctgccaccaa cctggcgctg agtgccctga acctggcaga gtgtgcaggg
     2221 gatgccgtgt ctgtggcgac gctggccgag atctatgtgg cggctgcatt gagagtgaag
     2281 accagtctcc cacgggcctt gcattttctg acacgcttct tcctgagcag tgcccgccag
     2341 gcctgcctgg cacagagtgg ctcagtgcct cctgccatgc agtggctctg ccaccccgtg
     2401 ggccaccgtt tcttcgtgga tggggactgg tccgtgctca gtaccccatg ggagagcctg
     2461 tacagcttgg ccgggaaccc agtggacccc ctggcccagg tgactcagct attccgggaa
     2521 catctcttag agcgagcact gaactgtgtg acccagccca accccagccc tgggtcagct
     2581 gatggggaca aggaattctc ggatgccctc gggtacctgc agctgctgaa cagctgttct
     2641 gatgctgcgg gggctcctgc ctacagcttc tccatcagtt ccagcatggc caccaccacc
     2701 ggcgtagacc cggtggccaa gtggtgggcc tctctgacag ctgtggtgat ccactggctg
     2761 cggcgggatg aggaggcggc tgagcggctg tgcccgctgg tggagcacct gccccgggtg
     2821 ctgcaggagt ctgagagacc cctgcccagg gcagctctgc actccttcaa ggctgcccgg
     2881 gccctgctgg gctgtgccaa ggcagagtct ggtccagcca gcctgaccat ctgtgagaag
     2941 gccagtgggt acctgcagga cagcctggct accacaccag ccagcagctc cattgacaag
     3001 gccgtgcagc tgttcctgtg tgacctgctt cttgtggtgc gcaccagcct gtggcggcag
     3061 cagcagcccc cggccccggc cccagcagcc cagggcacca gcagcaggcc ccaggcttcc
     3121 gcccttgagc tgcgtggctt ccaacgggac ctgagcagcc tgaggcggct ggcacagagc
     3181 ttccggcccg ccatgcggag ggtgttccta catgaggcca cggcccggct gatggcgggg
     3241 gccagcccca cacggacaca ccagctcctc gaccgcagtc tgaggcggcg ggcaggcccc
     3301 ggtggcaaag gaggcgcggt ggcggagctg gagccgcggc ccacgcggcg ggagcacgcg
     3361 gaggccttgc tgctggcctc ctgctacctg ccccccggct tcctgtcggc gcccgggcag
     3421 cgcgtgggca tgctggctga ggcggcgcgc acactcgaga agcttggcga tcgccggctg
     3481 ctgcacgact gtcagcagat gctcatgcgc ctgggcggtg ggaccactgt cacttccagc
     3541 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3601 ggaaaggacg aatattacta ttgatcgaca tacac
//
