LOCUS       sequence-283068-        3545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2863
FEATURES             Location/Qualifiers
     source          1..3545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgagcc acccttcagc gaggcggctt tggagcaggc gctgggcgag
       61 ccgtgcgatc tggacgcggc gctgctgacc gacatcgaag acatgcttca gcttatcaac
      121 aaccaagaca gtgacttccc tggcctattt gacccaccct atgctgggag tggggcaggg
      181 ggcacagacc ctgccagccc cgataccagc tccccaggca gcttgtctcc acctcctgcc
      241 acattgagct cctctcttga agccttcctg agcgggccgc aggcagcgcc ctcacccctg
      301 tcccctcccc agcctgcacc cactccattg aagatgtacc cgtccatgcc cgctttctcc
      361 cctgggcctg gtatcaagga agagtcagtg ccactgagca tcctgcagac ccccacccca
      421 cagcccctgc caggggccct cctgccacag agcttcccag ccccagcccc accgcagttc
      481 agctccaccc ctgtgttagg ctaccccagc cctccgggag gcttctctac aggaagccct
      541 cccgggaaca cccagcagcc gctgcctggc ctgccactgg cttccccgcc aggggtcccg
      601 cccgtctcct tgcacaccca ggtccagagt gtggtccccc agcagctact gacagtcaca
      661 gctgccccca cggcagcccc tgtaacgacc actgtgacct cgcagatcca gcaggtcccg
      721 gtcctgctgc agccccactt catcaaggca gactcgctgc ttctgacagc catgaagaca
      781 gacggagcca ctgtgaaggc ggcaggtctc agtcccctgg tctctggcac cactgtgcag
      841 acagggcctt tgccgaccct ggtgagtggc ggaaccatct tggcaacagt cccactggtc
      901 gtagatgcgg agaagctgcc tatcaaccgg ctcgcagctg gcagcaaggc cccggcctct
      961 gcccagagcc gtggagagaa gcgcacagcc cacaacgcca ttgagaagcg ctaccgctcc
     1021 tccatcaatg acaaaatcat tgagctcaag gatctggtgg tgggcactga ggcaaagctg
     1081 aataaatctg ctgtcttgcg caaggccatc gactacattc gctttctgca acacagcaac
     1141 cagaaactca agcaggagaa cctaagtctg cgcactgctg tccacaaaag caaatctctg
     1201 aaggatctgg tgtcggcctg tggcagtgga gggaacacag acgtgctcat ggagggcgtg
     1261 aagactgagg tggaggacac actgacccca cccccctcgg atgctggctc acctttccag
     1321 agcagcccct tgtcccttgg cagcaggggc agtggcagcg gtggcagtgg cagtgactcg
     1381 gagcctgaca gcccagtctt tgaggacagc aaggcaaagc cagagcagcg gccgtctctg
     1441 cacagccggg gcatgctgga ccgctcccgc ctggccctgt gcacgctcgt cttcctctgc
     1501 ctgtcctgca accccttggc ctccttgctg ggggcccggg ggcttcccag cccctcagat
     1561 accaccagcg tctaccatag ccctgggcgc aacgtgctgg gcaccgagag cagagatggc
     1621 cctggctggg cccagtggct gctgccccca gtggtctggc tgctcaatgg gctgttggtg
     1681 ctcgtctcct tggtgcttct ctttgtctac ggtgagccag tcacacggcc ccactcaggc
     1741 cccgccgtgt acttctggag gcatcgcaag caggctgacc tggacctggc ccggggagac
     1801 tttgcccagg ctgcccagca gctgtggctg gccctgcggg cactgggccg gcccctgccc
     1861 acctcccacc tggacctggc ttgtagcctc ctctggaacc tcatccgtca cctgctgcag
     1921 cgtctctggg tgggccgctg gctggcaggc cgggcagggg gcctgcagca ggactgtgct
     1981 ctgcgagtgg atgctagcgc cagcgcccga gacgcagccc tggtctacca taagctgcac
     2041 cagctgcaca ccatggggaa gcacacaggc gggcacctca ctgccaccaa cctggcgctg
     2101 agtgccctga acctggcaga gtgtgcaggg gatgccgtgt ctgtggcgac gctggccgag
     2161 atctatgtgg cggctgcatt gagagtgaag accagtctcc cacgggcctt gcattttctg
     2221 acacgcttct tcctgagcag tgcccgccag gcctgcctgg cacagagtgg ctcagtgcct
     2281 cctgccatgc agtggctctg ccaccccgtg ggccaccgtt tcttcgtgga tggggactgg
     2341 tccgtgctca gtaccccatg ggagagcctg tacagcttgg ccgggaaccc agtggacccc
     2401 ctggcccagg tgactcagct attccgggaa catctcttag agcgagcact gaactgtgtg
     2461 acccagccca accccagccc tgggtcagct gatggggaca aggaattctc ggatgccctc
     2521 gggtacctgc agctgctgaa cagctgttct gatgctgcgg gggctcctgc ctacagcttc
     2581 tccatcagtt ccagcatggc caccaccacc ggcgtagacc cggtggccaa gtggtgggcc
     2641 tctctgacag ctgtggtgat ccactggctg cggcgggatg aggaggcggc tgagcggctg
     2701 tgcccgctgg tggagcacct gccccgggtg ctgcaggagt ctgagagacc cctgcccagg
     2761 gcagctctgc actccttcaa ggctgcccgg gccctgctgg gctgtgccaa ggcagagtct
     2821 ggtccagcca gcctgaccat ctgtgagaag gccagtgggt acctgcagga cagcctggct
     2881 accacaccag ccagcagctc cattgacaag gccgtgcagc tgttcctgtg tgacctgctt
     2941 cttgtggtgc gcaccagcct gtggcggcag cagcagcccc cggccccggc cccagcagcc
     3001 cagggcacca gcagcaggcc ccaggcttcc gcccttgagc tgcgtggctt ccaacgggac
     3061 ctgagcagcc tgaggcggct ggcacagagc ttccggcccg ccatgcggag ggtgttccta
     3121 catgaggcca cggcccggct gatggcgggg gccagcccca cacggacaca ccagctcctc
     3181 gaccgcagtc tgaggcggcg ggcaggcccc ggtggcaaag gaggcgcggt ggcggagctg
     3241 gagccgcggc ccacgcggcg ggagcacgcg gaggccttgc tgctggcctc ctgctacctg
     3301 ccccccggct tcctgtcggc gcccgggcag cgcgtgggca tgctggctga ggcggcgcgc
     3361 acactcgaga agcttggcga tcgccggctg ctgcacgact gtcagcagat gctcatgcgc
     3421 ctgggcggtg ggaccactgt cacttccagc tagtaatgaa ctagtccgta acttgaaagt
     3481 atttcgattt cttggcttta tatatcttgt ggaaaggacg agttctgcca gccagtctca
     3541 ctccc
//
