LOCUS       sequence-283071-        2609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2866
FEATURES             Location/Qualifiers
     source          1..2609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtggccat ggacttgggc tctccctcgc tccctaagaa gagcctgcct
       61 gtccctgggg ccctggagca ggtggccagt cggctgagca gcaaagtggc tgcagaggtt
      121 cctcatggca gcaaacagga gctgcaggac ctcaaggccc agagcctgac cacctgcgag
      181 gtctgcggtg cctgctttga gacccgaaag ggcctgtcca gccacgcgcg ctcccacctg
      241 cggcagctgg gagtggcaga gtcggaaagc agcggcgcac ccatcgacct cctctacgag
      301 cttgtgaagc agaagggtct gcctgacgcc caccttgggc tgcccccagg cctggctaag
      361 aagtccagct cactgaagga ggtggtcgcc ggggcccccc ggcccggctt gctcagcctg
      421 gccaagccct tggatgcccc tgctgtcaac aaagccatca agtcgcctcc cggcttctcg
      481 gccaagggcc tgggccaccc gcccagctct ccactcctca aaaagacacc actggccctg
      541 gcgggctccc ctacccctaa gaatcctgag gacaagagcc cccagctgtc cctgagcccc
      601 cggccggcct ccccaaaggc acagtggcct cagtctgagg atgaggggcc cttgaacctc
      661 acctcaggcc cagagccagc acgagacatc cgctgcgagt tctgtggtga gttcttcgag
      721 aaccgcaagg gcctctcgag ccacgcgcgc tcccatctgc ggcaaatggg cgtgaccgag
      781 tggtacgtca atggctcgcc catcgacacg ctgcgggaga tcctgaagag acggacccag
      841 tctcggcctg gtggacctcc caacccacca gggccaagcc caaaagccct ggccaagatg
      901 atgggcggcg caggtcctgg cagctcactg gaagcccgca gcccctcgga ccttcacatc
      961 tcacccttgg ccaagaagtt gccaccacca ccgggcagcc ccctgggcca ctcaccaact
     1021 gcctctcctc ctcctacggc ccgaaagatg ttcccaggcc tggctgcacc ctccttgccc
     1081 aagaagctga agcctgaaca aatacgggtg gagatcaagc gggagatgct gccgggggcc
     1141 cttcatgggg aactgcaccc atctgagggt ccctgggggg caccacggga agacatgaca
     1201 cccctgaacc tgtcgtcccg ggcagagccg gtgcgcgaca tccgctgtga gttctgcggc
     1261 gagttcttcg agaaccgcaa gggcctgtcg agtcacgcgc gctcacacct gcggcagatg
     1321 ggtgtgaccg agtggtccgt caatggttcg cccatcgaca cactgcgaga gatcctcaag
     1381 aagaagtcca agccgtgcct catcaagaag gagccaccgg ctggagacct ggcccctgcc
     1441 ctggctgagg acgggcctcc caccgtggcc cctgggcccg tgcagtcccc actgccgctg
     1501 tcgcccctgg ctggccggcc aggcaaacca ggtgcagggc cggcccaggt tcctcgtgag
     1561 ctcagcctga cgcccatcac tggggccaag ccctcagcca ctggctacct gggctcagtg
     1621 gcagccaagc ggcccctgca ggaggaccgc ctcctcccag cagaggtcaa ggccaagacc
     1681 tacatccaga ctgaactgcc cttcaaggca aagacccttc atgagaagac ctcccactcc
     1741 tccaccgagg cctgctgcga gctgtgtggc ctttactttg aaaaccgcaa ggccctggcc
     1801 agccacgcac gggcacacct gcggcagttc ggcgtgaccg agtggtgcgt caatggctcg
     1861 cccatcgaga cactgagcga gtggatcaaa caccggcccc agaaggtggg cgcctaccgc
     1921 agctacatcc agggcggccg ccccttcacc aagaagttcc gcagtgccgg ccatggccgt
     1981 gacagtgaca agcggccgtc cctggggctg gcacccgggg gcctggccgt ggtcggccgc
     2041 agtgccggag gggagccagg gcccgaggct ggccgggcag ccgacggtgg tgagcggcct
     2101 ctggcagcca gcccgccagg caccgtgaag gctgaggagc accagcggca gaacatcaac
     2161 aaatttgaac gccgacaagc ccgccctcca gatgcctccg cagcccgggg aggcgaggac
     2221 accaatgacc tacagcagaa gctggaggag gtgcggcaac ccccaccccg agtccggcca
     2281 gtcccctccc tggtgccccg gcccccccag acatcacttg tcaagttcgt gggcaacatc
     2341 tacaccctca aatgcaggtt ctgtgaggtg gaattccagg gccccctctc catccaggaa
     2401 gagtgggtgc ggcacttaca gcggcacatc ctggagatga acttctccaa agcggacccc
     2461 ccacctgagg agtcccaggc cccgcaggca cagacagcgg cggcagaggc tccctagtaa
     2521 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2581 gacgagcgaa atgaggcata cgcgtgcat
//
