LOCUS       sequence-283074-        1961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2873
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgt tgtccaacag aacgacctag tatttgaatt tgctagtaac
       61 gtcatggagg atgaacgaca gcttggtgat ccagctattt ttcctgccgt aattgtggaa
      121 catgttcctg gtgctgatat tctcaatagt tatgccggtc tagcctgtgt ggaagagccc
      181 aatgacatga ttactgagag ttcactggat gttgctgaag aagaaatcat agacgatgat
      241 gatgatgaca tcacccttac agttgaagct tcttgtcatg acggggatga aacaattgaa
      301 actattgagg ctgctgaggc actcctcaat atggattccc ctggccctat gctggatgaa
      361 aaacgaataa ataataatat atttagttca cctgaagatg acatggttgt tgccccagtc
      421 acccatgtgt ccgtcacatt agatgggatt cctgaagtga tggaaacaca gcaggtgcaa
      481 gaaaaatatg cagactcacc gggagcctca tcaccagaac agcctaagag gaaaaaagga
      541 agaaaaacta aaccaccacg accagattcc ccagccacta cgccaaatat atctgtgaag
      601 aagaaaaaca aagatggaaa gggaaacaca atttatcttt gggagttttt actggcactg
      661 ctccaggaca aggctacttg tcctaaatac atcaagtgga cccagcgaga gaaaggcatt
      721 tttaaattgg tggattctaa agcagtgtcc aggttgtggg ggaagcacaa aaacaaacct
      781 gatatgaatt atgagaccat gggaagagca ctcaggtact attaccaaag gggtattctg
      841 gcaaaagtgg aaggtcagcg cttggtgtat cagtttaaag aaatgccaaa agatcttata
      901 tatataaatg atgaggatcc aagttccagc atagagtctt cagatccatc gctatcttca
      961 tcagccactt caaataggaa tcaaaccagc cggtcgagag tatcttcaag tccaggggta
     1021 aaaggaggag ccactacagt tctaaaacca gggaattcta aagctgcaaa acccaaagat
     1081 cctgtggaag ttgcacaacc atcagaagtt ttgaggacag tgcagcccac gcagtctcca
     1141 tatcctaccc agctcttccg gactgttcat gtagtacagc cagtacaggc tgtcccagag
     1201 ggagaagcag ctagaaccag taccatgcag gatgaaacat taaattcttc cgttcagagt
     1261 attaggacta tacaggctcc aacccaagtt ccagtggttg tgtctcctag gaatcagcag
     1321 ttgcatacag taacactcca aacagtgcca ctcacaacag ttatagccag cacagatcca
     1381 tcagcaggta ctggatctca gaagtttatt ttacaagcca ttccatcatc acagcccatg
     1441 acagtactga aagaaaatgt catgctgcag tcacaaaagg cgggctctcc tccttcaatt
     1501 gtcttgggcc ctgcccaggt tcagcaggtc cttactagca atgttcagac catttgcaat
     1561 ggaaccgtca gtgtggcttc ctctccatcc ttcagtgcta ctgcacctgt ggtgaccttt
     1621 tctcctcgca gttcacagct ggttgctcac ccacctggca ctgtaatcac ttcagttatc
     1681 aaaactcaag aaacaaaaac tcttacacag gaagtagaga aaaaggaatc tgaagatcat
     1741 ttgaaagaga acactgagaa aacggagcag cagccacagc cttatgtgat ggtagtgtcc
     1801 agttccaatg gatttacttc tcaggtagct atgaaacaaa acgaactgct ggaacccaac
     1861 tctttttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1921 tcttgtggaa aggacgacat atgtatatga catcacattc t
//
