LOCUS       sequence-283077-        1889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2874
FEATURES             Location/Qualifiers
     source          1..1889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgt tgtccaacag aacgacctag tatttgaatt tgctagtaac
       61 gtcatggagg atgaacgaca gcttggtgat ccagctattt ttcctgccgt aattgtggaa
      121 catgttcctg gtgctgatat tctcaatagt tatgccggtc tagcctgtgt ggaagagccc
      181 aatgacatga ttactgagag ttcactggat gttgctgaag aagaaatcat agacgatgat
      241 gatgatgaca tcacccttac agttgaagct tcttgtcatg acggggatga aacaattgaa
      301 actattgagg ctgctgaggc actcctcaat atggattccc ctggccctat gctggatgaa
      361 aaacgaataa atgggattcc tgaagtgatg gaaacacagc aggtgcaaga aaaatatgca
      421 gactcaccgg gagcctcatc accagaacag cctaagagga aaaaaggaag aaaaactaaa
      481 ccaccacgac cagattcccc agccactacg ccaaatatat ctgtgaagaa gaaaaacaaa
      541 gatggaaagg gaaacacaat ttatctttgg gagtttttac tggcactgct ccaggacaag
      601 gctacttgtc ctaaatacat caagtggacc cagcgagaga aaggcatttt taaattggtg
      661 gattctaaag cagtgtccag gttgtggggg aagcacaaaa acaaacctga tatgaattat
      721 gagaccatgg gaagagcact caggtactat taccaaaggg gtattctggc aaaagtggaa
      781 ggtcagcgct tggtgtatca gtttaaagaa atgccaaaag atcttatata tataaatgat
      841 gaggatccaa gttccagcat agagtcttca gatccatcgc tatcttcatc agccacttca
      901 aataggaatc aaaccagccg gtcgagagta tcttcaagtc caggggtaaa aggaggagcc
      961 actacagttc taaaaccagg gaattctaaa gctgcaaaac ccaaagatcc tgtggaagtt
     1021 gcacaaccat cagaagtttt gaggacagtg cagcccacgc agtctccata tcctacccag
     1081 ctcttccgga ctgttcatgt agtacagcca gtacaggctg tcccagaggg agaagcagct
     1141 agaaccagta ccatgcagga tgaaacatta aattcttccg ttcagagtat taggactata
     1201 caggctccaa cccaagttcc agtggttgtg tctcctagga atcagcagtt gcatacagta
     1261 acactccaaa cagtgccact cacaacagtt atagccagca cagatccatc agcaggtact
     1321 ggatctcaga agtttatttt acaagccatt ccatcatcac agcccatgac agtactgaaa
     1381 gaaaatgtca tgctgcagtc acaaaaggcg ggctctcctc cttcaattgt cttgggccct
     1441 gcccaggttc agcaggtcct tactagcaat gttcagacca tttgcaatgg aaccgtcagt
     1501 gtggcttcct ctccatcctt cagtgctact gcacctgtgg tgaccttttc tcctcgcagt
     1561 tcacagctgg ttgctcaccc acctggcact gtaatcactt cagttatcaa aactcaagaa
     1621 acaaaaactc ttacacagga agtagagaaa aaggaatctg aagatcattt gaaagagaac
     1681 actgagaaaa cggagcagca gccacagcct tatgtgatgg tagtgtccag ttccaatgga
     1741 tttacttctc aggtagctat gaaacaaaac gaactgctgg aacccaactc tttttagtaa
     1801 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1861 gacgagaagg ttggtgctcc agcgggtgc
//
