LOCUS       sequence-283080-        1217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2875
FEATURES             Location/Qualifiers
     source          1..1217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcaac ctgtgagatt agcaacattt ttagcaacta cttcagtgcg
       61 atgtacagct cggaggactc caccctggcc tctgttcccc ctgctgccac ctttggggcc
      121 gatgacttgg tactgaccct gagcaacccc cagatgtcat tggagggtac agagaaggcc
      181 agctggttgg gggaacagcc ccagttctgg tcgaagacgc aggttctgga ctggatcagc
      241 taccaagtgg agaagaacaa gtacgacgca agcgccattg acttctcacg atgtgacatg
      301 gatggcgcca ccctctgcaa ttgtgccctt gaggagctgc gtctggtctt tgggcctctg
      361 ggggaccaac tccatgccca gctgcgagac ctcacttcca gctcttctga tgagctcagt
      421 tggatcattg agctgctgga gaaggatggc atggccttcc aggaggccct agacccaggg
      481 ccctttgacc agggcagccc ctttgcccag gagctgctgg acgacggtca gcaagccagc
      541 ccctaccacc ccggcagctg tggcgcagga gccccctccc ctggcagctc tgacgtctcc
      601 accgcaggga ctggtgcttc tcggagctcc cactcctcag actccggtgg aagtgacgtg
      661 gacctggatc ccactgatgg caagctcttc cccagcgatg gttttcgtga ctgcaagaag
      721 ggggatccca agcacgggaa gcggaaacga ggccggcccc gaaagctgag caaagagtac
      781 tgggactgtc tcgagggcaa gaagagcaag cacgcgccca gaggcaccca cctgtgggag
      841 ttcatccggg acatcctcat ccacccggag ctcaacgagg gcctcatgaa gtgggagaat
      901 cggcatgaag gcgtcttcaa gttcctgcgc tccgaggctg tggcccaact atggggccaa
      961 aagaaaaaga acagcaacat gacctacgag aagctgagcc gggccatgag gtactactac
     1021 aaacgggaga tcctggaacg ggtggatggc cggcgactcg tctacaagtt tggcaaaaac
     1081 tcaagcggct ggaaggagga agaggttctc cagagtcgga actagtaatg aactagtccg
     1141 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgactaacag
     1201 aatggctggc caactta
//
