LOCUS       sequence-283083-        1667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2877
FEATURES             Location/Qualifiers
     source          1..1667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacgtc tctgcatgag ggacccacga accagctgga tctgctcatc
       61 cgggccgtgg aagcatcagt tcacagcagt aatgcacact gtacagataa gacaattgaa
      121 gctgctgaag ccctgcttca tatggaatct cctacctgct tgagggattc aagaagtcct
      181 gaattcatcc atgctgctat gaggccagat gtcattacag aaactgtagt ggaggtgtca
      241 actgaagagt ctgaacccat ggatacctct cctattccaa catcaccaga tagccatgaa
      301 ccaatgaaaa agaaaaaagt tggccgtaaa ccaaagaccc agcaatcacc aatttccaat
      361 gggtctcctg agttaggtat aaagaagaaa ccaagagaag gaaaaggaaa cacaacctat
      421 ttgtgggagt ttcttttaga tctacttcaa gataaaaata cttgtcccag gtatattaaa
      481 tggactcaga gagaaaaagg catattcaag ctggtggatt caaaggctgt ctctaagctt
      541 tggggaaagc ataagaacaa accagacatg aactatgaaa ccatgggacg agctttgaga
      601 tactactacc aaaggggaat tcttgcaaag gttgaaggac agaggcttgt atatcagttc
      661 aaggatatgc cgaaaaacat agtggtcata gatgatgaca aaagtgaaac ctgtaatgaa
      721 gatttagcag gaactactga tgaaaaatca ttagaacgag tgtcactgtc tgcagaaagt
      781 ctcctgaaag cagcatcctc tgttcgcagt ggaaaaaatt catcccctat aaactgctcc
      841 agagcagaga agggtgtagc tagagttgtg aatatcactt cccctgggca cgatgcttca
      901 tccaggtctc ctactaccac tgcatctgtg tcagcaacag cagctccaag gacagttcgt
      961 gtggcaatgc aggtacctgt tgtaatgaca tcattgggtc agaaaatttc aactgtggca
     1021 gttcagtcag ttaatgcagg tgcaccatta ataaccagca ctagtccaac aacagcgacc
     1081 tctccaaagg tagtcattca gacaatccct actgtgatgc cagcttctac tgaaaatgga
     1141 gacaaaatca ccatgcagcc tgccaaaatt attaccatcc cagctacaca gcttgcacag
     1201 tgtcaactgc agacaaagtc aaatctgact ggatcaggaa gcattaacat tgttggaacc
     1261 ccattggctg tgagagcact tacccctgtt tcaatagccc atggtacacc tgtaatgaga
     1321 ctatcaatgc ctactcagca ggcatctggc cagactcctc ctcgagttat cagtgcagtc
     1381 ataaaggggc cagaggttaa atcggaagca gtggcaaaaa agcaagaaca tgatgtgaaa
     1441 actttgcagc tagtagaaga aaaaccagca gatggaaata agacagtgac ccacgtagtg
     1501 gttgtcagtg cgccttcagc tattgccctt cctgtaacta tgaaaacaga aggactagtg
     1561 acatgtgaga aatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1621 tatatatctt gtggaaagga cgagactctg gcgccagagc ttcacgt
//
